Enhancing Preoperative Diagnosis Accuracy of Stage III Gastric Cancer with Circulating circRNAs.

IF 3.4 2区 医学 Q2 ONCOLOGY
Annals of Surgical Oncology Pub Date : 2025-01-01 Epub Date: 2024-10-21 DOI:10.1245/s10434-024-16387-2
Koichi Matsutoka, Katsutoshi Shoda, Yudai Higuchi, Takashi Nakayama, Ryo Saito, Suguru Maruyama, Koichi Takiguchi, Yuki Nakata, Shinji Furuya, Kensuke Shiraishi, Yoshihiko Kawaguchi, Hidetake Amemiya, Kiyoshi Masuda, Daisuke Ichikawa
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引用次数: 0

Abstract

Background: The prognosis remains poor for stage III gastric cancer, and neoadjuvant chemotherapy is increasingly used to improve outcomes. Accurate diagnosis prior to treatment is essential to develop appropriate treatment strategies for poor prognosis subgroups. This study aims to enhance the accuracy of pre-treatment gastric cancer diagnosis using a biological approach centered on circulating circular RNA (circRNA).

Materials and methods: We conducted a comprehensive analysis of circRNA expression profiles using two Gene Expression Omnibus datasets to identify circRNA candidates associated with stage III gastric cancer. Subsequently, we validated these circRNA biomarkers in two independent clinical cohorts comprising a total of 174 patients with gastric cancer and non-disease controls through real-time polymerase chain reaction (PCR).

Results: Genome-wide circRNA analysis identified a panel of four biomarkers capable of diagnosing pathologically confirmed stage III (pStage III) gastric cancer. In a training cohort (n = 83), a clinically applicable panel of four circRNAs was developed (AUC 0.81), which was successfully validated in an independent clinical cohort (n = 82; AUC 0.76). To assess clinical utility, we combined clinical imaging (cStage) with the circRNA panel. Among those initially diagnosed as cStage III but later confirmed as pStage I/II, 86% were accurately diagnosed using the molecular biological approach with circRNAs.

Conclusions: We have developed a circRNA-based non-invasive liquid biopsy that can improve the diagnostic performance of pStage III gastric cancer before treatment. Our circRNA model could provide a sophisticated and personalized approach to assist in treatment planning for patients with advanced gastric cancer.

利用循环 circRNA 提高 III 期胃癌的术前诊断准确性
背景:III 期胃癌的预后仍然较差,为改善预后,越来越多地采用新辅助化疗。治疗前的准确诊断对于为预后较差的亚组制定适当的治疗策略至关重要。本研究旨在利用以循环 RNA(circRNA)为中心的生物学方法提高胃癌治疗前诊断的准确性:我们利用两个基因表达总库数据集对循环 RNA 表达谱进行了全面分析,以确定与 III 期胃癌相关的循环 RNA 候选者。随后,我们通过实时聚合酶链反应(PCR)在两个独立的临床队列中验证了这些 circRNA 生物标记物,这两个临床队列由总共 174 名胃癌患者和非疾病对照组组成:结果:全基因组循环RNA分析确定了能够诊断病理确诊的III期(pStage III)胃癌的四种生物标记物。在一个训练队列(n = 83)中,开发出了一个临床适用的四种 circRNA 小组(AUC 0.81),该小组在一个独立的临床队列(n = 82;AUC 0.76)中得到了成功验证。为了评估临床实用性,我们将临床成像(cStage)与 circRNA 面板相结合。在最初诊断为c分期III但后来确诊为p分期I/II的患者中,86%的患者通过circRNAs分子生物学方法得到了准确诊断:我们开发了一种基于 circRNA 的无创液体活检方法,它可以提高 pStage III 胃癌治疗前的诊断率。我们的循环RNA模型可以提供一种复杂的个性化方法,帮助晚期胃癌患者制定治疗计划。
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来源期刊
CiteScore
5.90
自引率
10.80%
发文量
1698
审稿时长
2.8 months
期刊介绍: The Annals of Surgical Oncology is the official journal of The Society of Surgical Oncology and is published for the Society by Springer. The Annals publishes original and educational manuscripts about oncology for surgeons from all specialities in academic and community settings.
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