Mechanisms of Xuefu Zhuyu Tang in the Treatment of Diabetic Erectile Dysfunction in Rats Through the Regulation of Vascular Endothelial Function by CaSR/PLC/PKC and MEK/ERK/RSK Pathways.

IF 2.1 4区 医学 Q3 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH
Xiao Li, Mingzhao Zhang, Junlong Feng, Jisheng Wang, Kaifeng Wang, Baojun Ju, Xiangyu Wang, Guoming Pang
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Abstract

Xuefu zhuyu Tang (XFZYT) is a classic formula used for promoting blood circulation and resolving blood stasis in Traditional Chinese Medicine. Clinical data have indicated that XFZYT plays a significant therapeutic role in diabetes-induced erectile dysfunction (DIED) disease, but the underlying mechanism remains elusive. Male Sprague-Dawley (SD) rats were randomly categorized into normal, model, and treatment groups. The diabetic rat model was established via intraperitoneal injection of streptozotocin. DIED rats were screened using apomorphine, and the number of erections was measured after 8 weeks of XFZYT treatment. Serum nitric oxide (NO) and endothelin-1 levels as well as penile tissue structure alterations were assessed by hematoxylin-eosin staining and electron microscopy. CaSR/PLC/PKC and MEK/ERK/RSK pathway-related proteins in the penile tissue were detected by western blotting (WB) analysis and polymerase chain reaction (PCR). Compared with the blank group, the model group rats showed a significant decrease in weight and erectile function. The pathological damage in the penile tissues of the model rats was indicated by a significantly decreased serum NO level and an increased endothelin-1 content. After treatment with XFZYT, the protein expression of CaSR, PLCβ1, PKCβ, MEK1, ERK1, and RSK1 in the penile tissue was significantly increased. Overall, the treatment group showed significant improvements in the evaluated indexes. In conclusion, this study revealed that XFZYT improves erectile function in diabetic rats, and the underlying mechanism might be linked with the regulation of CaSR/PLC/PKC and related molecules of the MEK/ERK/RSK pathway, which promotes the vascular endothelial diastolic effect.

学府指南汤通过CaSR/PLC/PKC和MEK/ERK/RSK途径调控血管内皮功能治疗糖尿病大鼠勃起功能障碍的机制
血府逐瘀汤(XFZYT)是中医活血化瘀的经典方剂。临床数据表明,XFZYT 对糖尿病诱发的勃起功能障碍(DIED)疾病有显著的治疗作用,但其潜在的机制仍不清楚。雄性 Sprague-Dawley (SD) 大鼠被随机分为正常组、模型组和治疗组。通过腹腔注射链脲佐菌素建立糖尿病大鼠模型。用阿朴吗啡筛选 DIED 大鼠,XFZYT 治疗 8 周后测量勃起次数。血清一氧化氮(NO)和内皮素-1水平以及阴茎组织结构的改变均通过苏木精-伊红染色和电子显微镜进行了评估。阴茎组织中的CaSR/PLC/PKC和MEK/ERK/RSK通路相关蛋白通过Western印迹(WB)分析和聚合酶链反应(PCR)进行检测。与空白组相比,模型组大鼠的体重和勃起功能明显下降。模型组大鼠阴茎组织的病理损伤表现为血清 NO 水平显著下降和内皮素-1 含量增加。经 XFZYT 治疗后,阴茎组织中 CaSR、PLCβ1、PKCβ、MEK1、ERK1 和 RSK1 的蛋白表达量明显增加。总体而言,治疗组的各项评估指标均有明显改善。总之,本研究揭示了XFZYT能改善糖尿病大鼠的勃起功能,其潜在机制可能与调控CaSR/PLC/PKC和MEK/ERK/RSK通路的相关分子,从而促进血管内皮舒张效应有关。
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来源期刊
American Journal of Men's Health
American Journal of Men's Health PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH-
CiteScore
3.70
自引率
4.30%
发文量
107
审稿时长
15 weeks
期刊介绍: American Journal of Men"s Health will be a core resource for cutting-edge information regarding men"s health and illness. The Journal will publish papers from all health, behavioral and social disciplines, including but not limited to medicine, nursing, allied health, public health, health psychology/behavioral medicine, and medical sociology and anthropology.
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