Augmentation of antifungal activity of fluconazole using a clove oil nanoemulgel formulation optimized by factorial randomized D-optimal design.

IF 2.6 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
3 Biotech Pub Date : 2024-11-01 Epub Date: 2024-10-18 DOI:10.1007/s13205-024-04116-1
Shaimaa M Badr-Eldin, Hibah Mubarak Aldawsari, Sabna Kotta, Mahmoud Abdelkhalek Elfaky
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引用次数: 0

Abstract

In the present study, fluconazole (FLU) showed the highest solubility in clove oil and was selected as the oil phase for the FLU-loaded nanoemulsion (FLU-NE). Among the studied cosurfactants, Labrafac was better than ethanol at providing globules with acceptable sizes and a lower polydispersity index (PDI) when Tween 80 was the surfactant. This optimized FLU-NE was thermodynamically stable. Furthermore, FLU-NE stored at 40 ± 2 °C and 75 ± 5% relative humidity for 6 months demonstrated good stability. The FLU-NE was converted to a FLU-loaded nanoemulsion gel (FLU-NEG) using 2% w/v sodium carboxymethyl cellulose. The FLU-NEG was acceptable in terms of visual appearance and spreadability. Rheological studies revealed pseudoplastic behavior for FLU-NEG. The viscosity of FLU-NEG decreased when the applied rpm was increased. FLU-NEG showed greater drug release than that from a FLU-GEL formulation. Furthermore, the FLU release from FLU-NEG followed the Higuchi model. The results from the in vitro antifungal evaluation of FLU-NEG on Candida albicans ATCC 76615 strain confirmed the increase in the antifungal activity of FLU by clove oil. Significant differences were observed in the zones of inhibition produced by FLU-NEG compared to those produced by the blank nanoemulsion gel (B-NEG), fluconazole suspension (FLU-SUS), and nystatin samples. Thus, the increase in the antifungal activity of FLU using clove oil as the oil phase in its nanoemulsion formulation was quite evident from our results. Therefore, the developed FLU-NEG could be considered a potential candidate for further preclinical and clinical studies.

使用丁香油纳米凝胶配方增强氟康唑的抗真菌活性,该配方通过因子随机 D-优化设计进行了优化。
在本研究中,氟康唑(FLU)在丁香油中的溶解度最高,因此被选为氟康唑负载纳米乳液(FLU-NE)的油相。在所研究的共表面活性剂中,当 Tween 80 作为表面活性剂时,Labrafac 在提供具有可接受尺寸和较低多分散指数(PDI)的球状物方面优于乙醇。这种优化的 FLU-NE 具有热力学稳定性。此外,FLU-NE 在 40 ± 2 °C 和 75 ± 5%相对湿度条件下储存 6 个月后显示出良好的稳定性。使用 2% w/v 羧甲基纤维素钠将 FLU-NE 转化为 FLU 负载纳米乳液凝胶(FLU-NEG)。FLU-NEG 在外观和涂抹性方面均可接受。流变学研究表明,FLU-NEG 具有假塑性行为。当转速增加时,FLU-NEG 的粘度降低。与 FLU-GEL 制剂相比,FLU-NEG 的药物释放量更大。此外,FLU-NEG 的 FLU 释放遵循樋口模型。FLU-NEG 对白色念珠菌 ATCC 76615 株的体外抗真菌评价结果证实,丁香油提高了 FLU 的抗真菌活性。与空白纳米乳凝胶(B-NEG)、氟康唑悬浮剂(FLU-SUS)和奈司他丁样品相比,FLU-NEG 产生的抑菌区有显著差异。因此,从我们的研究结果来看,以丁香油作为纳米乳液配方中的油相,FLU 的抗真菌活性明显提高。因此,所开发的 FLU-NEG 可被视为进一步临床前和临床研究的潜在候选药物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
3 Biotech
3 Biotech Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)
CiteScore
6.00
自引率
0.00%
发文量
314
期刊介绍: 3 Biotech publishes the results of the latest research related to the study and application of biotechnology to: - Medicine and Biomedical Sciences - Agriculture - The Environment The focus on these three technology sectors recognizes that complete Biotechnology applications often require a combination of techniques. 3 Biotech not only presents the latest developments in biotechnology but also addresses the problems and benefits of integrating a variety of techniques for a particular application. 3 Biotech will appeal to scientists and engineers in both academia and industry focused on the safe and efficient application of Biotechnology to Medicine, Agriculture and the Environment.
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