A selective mitochondria-targeted fluorescent probe for imaging cysteine in drug-induced liver injury.

IF 5.6 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta Pub Date : 2025-01-01 Epub Date: 2024-10-16 DOI:10.1016/j.talanta.2024.127056

Guixin Qin, Lingli Gao, Nan Yin, Mingxiu Wang, Yuting Wang, Jiali Tang, Jianhua Gong, Qingling Xu

引用次数: 0

Abstract

Cysteine (Cys) is involved in many physiological processes. It's challenging to detect Cys selectively as it has similar chemical structure with other biothiols such as homocysteine (Hcy) and glutathione (GSH). In this work, a novel fluorescence probe toward mitochondrial cysteine, HPXI-6C, has been developed by employing carbonate as a new recognizing unit and hemicyanine as a chromophore. HPXI-6C exhibits a high selectivity to Cys over hydrogen sulfide, homocysteine and glutathione. The limit of detection toward Cys was determined to be 42 nM. HPXI-6C can localize in mitochondria and produce strong fluorescence peaked at 725 nm in response to Cys in tumor cells. The uptake and generation pathways of Cys in acetaminophen hepatotoxicity cells was revealed by using HPXI-6C. HPXI-6C has been successfully applied in imaging of Cys in drug-induced liver injury in vivo. The research demonstrated that HPXI-6C is powerful in monitoring Cys and is conducive to the early diagnosis of drug-induced liver injury diseases.

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一种选择性线粒体靶向荧光探针，用于成像药物诱导肝损伤中的半胱氨酸。

半胱氨酸（Cys）参与许多生理过程。由于半胱氨酸与同型半胱氨酸（Hcy）和谷胱甘肽（GSH）等其他生物硫醇具有相似的化学结构，因此选择性地检测半胱氨酸具有挑战性。在这项工作中，通过使用碳酸盐作为新的识别单元和半氰胺作为发色团，开发出了一种新型线粒体半胱氨酸荧光探针 HPXI-6C。与硫化氢、高半胱氨酸和谷胱甘肽相比，HPXI-6C 对 Cys 具有很高的选择性。对 Cys 的检测限被确定为 42 nM。HPXI-6C 能在线粒体中定位，并在 725 纳米波长处对肿瘤细胞中的 Cys 产生强荧光峰值。利用 HPXI-6C 揭示了对乙酰氨基酚肝毒性细胞对 Cys 的吸收和生成途径。HPXI-6C 已成功应用于体内药物性肝损伤中 Cys 的成像。研究表明，HPXI-6C 在监测 Cys 方面功能强大，有利于药物性肝损伤疾病的早期诊断。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Talanta 化学-分析化学

CiteScore

12.30

自引率

4.90%

发文量

861

审稿时长

29 days

期刊介绍： Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.