Multienzyme-like active MnO2 nanozyme with ROS scavenging for inflammatory injury therapy induced by avian flavivirus through antiviral function.

IF 5.4 2区 医学 Q1 BIOPHYSICS
Linhua Xu, Wei Fan, Ming Han, Wei Li, Yu He, Zhen Wu, Aimin Wu, Yue Xie, Huaiyong Gao, Shun Chen, Xianxiang Wang
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引用次数: 0

Abstract

Duck Tembusu virus (DTMUV) is an acute avian flavivirus that primarily infects poultry, mosquitoes, and some mammals including humans. The viral infection triggers reactive oxygen species (ROS) and inflammatory response that are crucial in mediating injury. Crafting multifunctional nanozymes that possess both ROS scavenging and anti-inflammatory activities presents formidable challenges. The study synthesized manganese dioxide cauliflowers (MnO2 Cfs) endowed with multiple enzyme-like activities (analogous to SOD, CAT, and GPX) that effectively alleviated the injury induced by DTMUV both in vitro and in vivo. Remarkably, MnO2 Cfs efficiently neutralized various ROS, encompassing hydrogen peroxide (H2O2), superoxide anion (O2·-) and hydroxyl radical (·OH). Our in vitro assessments showed that MnO2 Cfs could alleviate cytopathic effects and modulate the innate immune response during DTMUV infection through their ROS scavenging and anti-inflammatory properties. In vivo experiments supported these findings, demonstrating that ducklings therapied by MnO2 Cfs experienced alleviated injury during DTMUV infection. Importantly, MnO2 Cfs also effectively inhibited DTMUV replication in both laboratory and field conditions. This study presents a novel strategy for nanozyme design, promising significant therapeutic potential for treating viral inflammatory diseases.

具有清除 ROS 功能的多酶活性 MnO2 纳米酶,通过抗病毒功能治疗禽黄病毒诱发的炎症损伤。
鸭丹布苏病毒(DTMUV)是一种急性禽流感病毒,主要感染家禽、蚊子和包括人类在内的一些哺乳动物。病毒感染会引发活性氧(ROS)和炎症反应,这对介导损伤至关重要。制作同时具有清除 ROS 和抗炎活性的多功能纳米酶是一项艰巨的挑战。该研究合成了具有多种酶类活性(类似于 SOD、CAT 和 GPX)的二氧化锰菜花(MnO2 Cfs),可有效缓解 DTMUV 在体外和体内诱导的损伤。值得注意的是,MnO2 Cfs 能有效中和各种 ROS,包括过氧化氢(H2O2)、超氧阴离子(O2--)和羟自由基(-OH)。我们的体外评估结果表明,MnO2 Cfs具有清除ROS和抗炎特性,可在DTMUV感染期间减轻细胞病理效应并调节先天免疫反应。体内实验也证实了这些发现,表明使用二氧化锰 Cfs 治疗的雏鸭在 DTMUV 感染期间受到的伤害有所减轻。重要的是,二氧化锰 Cfs 还能在实验室和野外条件下有效抑制 DTMUV 的复制。这项研究提出了一种新的纳米酶设计策略,有望为治疗病毒性炎症疾病带来巨大的治疗潜力。
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来源期刊
Colloids and Surfaces B: Biointerfaces
Colloids and Surfaces B: Biointerfaces 生物-材料科学:生物材料
CiteScore
11.10
自引率
3.40%
发文量
730
审稿时长
42 days
期刊介绍: Colloids and Surfaces B: Biointerfaces is an international journal devoted to fundamental and applied research on colloid and interfacial phenomena in relation to systems of biological origin, having particular relevance to the medical, pharmaceutical, biotechnological, food and cosmetic fields. Submissions that: (1) deal solely with biological phenomena and do not describe the physico-chemical or colloid-chemical background and/or mechanism of the phenomena, and (2) deal solely with colloid/interfacial phenomena and do not have appropriate biological content or relevance, are outside the scope of the journal and will not be considered for publication. The journal publishes regular research papers, reviews, short communications and invited perspective articles, called BioInterface Perspectives. The BioInterface Perspective provide researchers the opportunity to review their own work, as well as provide insight into the work of others that inspired and influenced the author. Regular articles should have a maximum total length of 6,000 words. In addition, a (combined) maximum of 8 normal-sized figures and/or tables is allowed (so for instance 3 tables and 5 figures). For multiple-panel figures each set of two panels equates to one figure. Short communications should not exceed half of the above. It is required to give on the article cover page a short statistical summary of the article listing the total number of words and tables/figures.
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