RNA sequencing and functional analysis uncover key long non-coding RNAs involved in regulating pollen fertility during the process of gametocidal action in wheat.

IF 6.2 1区 生物学 Q1 PLANT SCIENCES
Wenrui Zhang, Dan Wang, Zhonghuan Yin, Lu Tang, Xiaoyang Pan, Changhong Guo
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Abstract

Gametocidal (Gc) chromosomes have been widely utilized in genetic breeding due to their ability to induce chromosomal breakage and eliminate gametes that lack them. Long noncoding RNAs (lncRNAs) have various functional mechanisms in regulating pollen and anther development; however, their regulatory contributions to Gc action are still unknown. Here, we identified 2824 differentially expressed lncRNAs (DE-lncRNAs) from the anther tissues of Triticum aestivum cv. Chinese Spring (CS) and Chinese Spring-Gc 3C chromosome monosomic addition line (CS-3C) through sequencing. In this study, we predicted 161 target mRNAs for 145 DE-lncRNAs, including 104 cis-regulatory, 60 trans-regulatory, and three both cis-regulatory and trans-regulatory manner. Combined with our previous miRNA sequencing data, 241 DE-lncRNAs functioned as potential endogenous target mimics (eTMs) for 84 differentially expressed microRNAs (DE-miRNAs, including 12 novel miRNAs). The results of transient transformation in tobacco leaves indicated that L006278 could bind to MTCONS_00006277, which encoded a calcineurin CBL-interacting protein kinase 19-like, and suppress its expression. Furthermore, L117735 could function as an eTM for tae-miR9657b-3p, and L056972 could function as an eTM for gc-m2240-5p. To explore the function of lncRNAs in the process of Gc action, we transformed L006278, an up-regulated lncRNA in CS-3C, into rice to analyze its effect on pollen fertility. Overexpression of L006278 led to a reduction in rice pollen fertility. Overall, our findings indicate that lncRNAs can contribute to the regulation of pollen fertility during the process of Gc action by regulating the expression levels of target mRNAs and acting as eTMs for certain key miRNAs.

RNA 测序和功能分析发现了在小麦配子作用过程中参与调控花粉育性的关键长非编码 RNA。
杀配子(Gc)染色体能够诱导染色体断裂并消除缺乏该染色体的配子,因此已被广泛用于遗传育种。长非编码 RNA(lncRNA)在调控花粉和花药发育方面具有多种功能机制;然而,它们对 Gc 作用的调控贡献仍然未知。在此,我们通过测序从中国春(CS)和中国春-Gc 3C 染色体单体加系(CS-3C)的花药组织中鉴定了 2824 个差异表达的 lncRNAs(DE-lncRNAs)。在这项研究中,我们预测了 145 个 DE-lncRNA 的 161 个靶 mRNA,包括 104 个顺式调控、60 个反式调控和 3 个同时具有顺式调控和反式调控方式的靶 mRNA。结合我们以前的 miRNA 测序数据,241 个 DE-lncRNAs 可作为 84 个差异表达 microRNAs(DE-miRNAs,包括 12 个新型 miRNAs)的潜在内源靶标模拟物(eTMs)。烟草叶片的瞬时转化结果表明,L006278能与编码钙调蛋白激酶CBL相互作用蛋白激酶19-like的MTCONS_00006277结合并抑制其表达。此外,L117735 可作为 tae-miR9657b-3p 的 eTM,L056972 可作为 gc-m2240-5p 的 eTM。为了探索lncRNA在Gc作用过程中的功能,我们将CS-3C中上调的lncRNA L006278转化到水稻中,分析其对花粉育性的影响。L006278的过表达导致水稻花粉育性降低。总之,我们的研究结果表明,在Gc作用过程中,lncRNA可通过调节目标mRNA的表达水平和充当某些关键miRNA的eTMs来促进花粉育性的调控。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
The Plant Journal
The Plant Journal 生物-植物科学
CiteScore
13.10
自引率
4.20%
发文量
415
审稿时长
2.3 months
期刊介绍: Publishing the best original research papers in all key areas of modern plant biology from the world"s leading laboratories, The Plant Journal provides a dynamic forum for this ever growing international research community. Plant science research is now at the forefront of research in the biological sciences, with breakthroughs in our understanding of fundamental processes in plants matching those in other organisms. The impact of molecular genetics and the availability of model and crop species can be seen in all aspects of plant biology. For publication in The Plant Journal the research must provide a highly significant new contribution to our understanding of plants and be of general interest to the plant science community.
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