Hydrogen Sulfide (H₂S) Activatable Photodynamic Therapy Against Colon Cancer by Tunable FRET Effect.

IF 3.5 3区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY

Chemistry - An Asian Journal Pub Date : 2025-01-02 Epub Date: 2024-11-14 DOI:10.1002/asia.202400840

Mengting Zhu, Baoxuan Huang, Guoliang Yang, Weian Zhang, Jia Tian

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引用次数: 0

Abstract

Developing activatable photodynamic agents is becoming a promising mode for realizing selective photodynamic therapy (PDT) in cancer treatment. However, till now only a few H₂S-activatable photodynamic agents have been involved in this field. Here, we fabricated H₂S-activatable nano-assemblies (P@TPPCY) for the treatment of colon cancer containing high concentration H₂S. The H₂S-activatable photosensitizer (TPPCY) was synthesized by covalent conjugation between tetraphenylporphyrin (TPP) and heptamethine cyanine (Cy7), and then TPPCY was encapsulated by an amphiphilic polymer DSPE-mPEG to form P@TPPCY nano-assemblies. We demonstrated that the photosensitizing effect of TPP was efficiently quenched by Cy7 with strong absorption in the NIR region via fluorescence resonance energy transfer (FRET) effect. Interestingly, the FRET effect between Cy7 and TPP was attenuated by the decrease of absorption of Cy7 in the NIR region after the Cy7 reacted with H₂S, and then the photosensitizing effect of TPP in P@TPPCY was activated. Strikingly, the P@TPPCY showed efficient H₂S-activatable photodynamic therapy (PDT) in vitro against HCT116 cells (human colon carcinoma cell line), thus this work provides a new method for realizing accurate treatment of colon cancer in virtue of high H₂S concentration microenvironment.

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利用可调 FRET 效应的硫化氢 (H2S) 激活光动力疗法治疗结肠癌。

开发可激活的光动力药剂正成为实现癌症治疗中选择性光动力疗法（PDT）的一种前景广阔的模式。然而，迄今为止，只有少数 H2S 可激活光动力制剂涉足这一领域。在此，我们制备了可激活 H2S 的纳米组件（P@TPPCY），用于治疗含有高浓度 H2S 的结肠癌。通过四苯基卟啉（TPP）和七氨基氰（Cy7）共价共轭合成了可激活 H2S 的光敏剂（TPPCY），然后用两亲性聚合物 DSPE-mPEG 将 TPPCY 包覆成 P@TPPCY 纳米组装体。我们证明，通过荧光共振能量转移（FRET）效应，在近红外区域具有强吸收的 Cy7 能有效淬灭 TPP 的光敏效应。有趣的是，Cy7 与 H2S 反应后，Cy7 在近红外区域的吸收减少，从而削弱了 Cy7 与 TPP 之间的 FRET 效应，进而激活了 P@TPPCY 中 TPP 的光敏效应。令人瞩目的是，P@TPPCY 在体外对 HCT116 细胞（人类结肠癌细胞系）表现出了高效的 H2S 激活型光动力疗法（PDT），从而为在高 H2S 浓度微环境下实现结肠癌的精准治疗提供了一种新方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Chemistry - An Asian Journal 化学-化学综合

CiteScore

7.00

自引率

2.40%

发文量

535

审稿时长

1.3 months

期刊介绍： Chemistry—An Asian Journal is an international high-impact journal for chemistry in its broadest sense. The journal covers all aspects of chemistry from biochemistry through organic and inorganic chemistry to physical chemistry, including interdisciplinary topics. Chemistry—An Asian Journal publishes Full Papers, Communications, and Focus Reviews. A professional editorial team headed by Dr. Theresa Kueckmann and an Editorial Board (headed by Professor Susumu Kitagawa) ensure the highest quality of the peer-review process, the contents and the production of the journal. Chemistry—An Asian Journal is published on behalf of the Asian Chemical Editorial Society (ACES), an association of numerous Asian chemical societies, and supported by the Gesellschaft Deutscher Chemiker (GDCh, German Chemical Society), ChemPubSoc Europe, and the Federation of Asian Chemical Societies (FACS).