Electrospinning Recombinant Spider Silk Fibroin-Reinforced PLGA Membranes: A Biocompatible Scaffold for Wound Healing Applications.

IF 5.4 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Biomaterials Science & Engineering Pub Date : 2024-11-11 Epub Date: 2024-10-22 DOI:10.1021/acsbiomaterials.4c01605

Yuanyuan Chen, Emma J Murphy, Zhi Cao, Ciara Buckley, Yvonne Cortese, Bor Shin Chee, Thomas Scheibel

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引用次数: 0

Abstract

Polylactide-polyglycolide (PLGA) is one of the most attractive polymeric biomaterials used to fabricate medical devices for drug delivery and tissue engineering applications. Nevertheless, the utilization of PLGA in load-bearing applications is restricted due to its inadequate mechanical properties. This study examines the potential of recombinant silk fibroin (eADF4), a readily producible biomaterial, as a reinforcing agent for PLGA. The PLGA/eADF4 composite membranes were developed by using the process of electrospinning. The spinnability of the electrospinning solutions and the physicochemical, mechanical, and thermal properties of the composite membranes were characterized. The addition of eADF4 increased the viscosity of the electrospinning solutions and enhanced both the mechanical characteristics and the thermal stability of the composites. This study demonstrates that PLGA membranes reinforced with recombinant spider silk fibroin are noncytotoxic, significantly enhance cell migration and wound closure, and do not trigger an inflammatory response, making them ideal candidates for advanced wound healing applications.

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电纺丝重组蜘蛛丝纤维蛋白增强聚乳酸乙二醛（PLGA）膜：用于伤口愈合的生物相容性支架。

聚乳酸-聚乙二醇（PLGA）是最具吸引力的聚合物生物材料之一，可用于制造药物输送和组织工程应用的医疗器械。然而，由于其机械性能不足，PLGA 在承重应用中的使用受到了限制。本研究探讨了重组蚕丝纤维素（eADF4）作为 PLGA 增强剂的潜力。PLGA/eADF4 复合膜是通过电纺丝工艺制成的。研究人员对电纺溶液的可纺性以及复合膜的物理化学、机械和热性能进行了表征。添加 eADF4 增加了电纺丝溶液的粘度，提高了复合材料的机械特性和热稳定性。这项研究表明，用重组蜘蛛丝纤维蛋白增强的聚乳酸乙丙橡胶（PLGA）膜无细胞毒性，能显著促进细胞迁移和伤口闭合，而且不会引发炎症反应，是先进伤口愈合应用的理想候选材料。

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来源期刊

ACS Biomaterials Science & Engineering Materials Science-Biomaterials

CiteScore

10.30

自引率

3.40%

发文量

413

期刊介绍： ACS Biomaterials Science & Engineering is the leading journal in the field of biomaterials, serving as an international forum for publishing cutting-edge research and innovative ideas on a broad range of topics: Applications and Health – implantable tissues and devices, prosthesis, health risks, toxicology Bio-interactions and Bio-compatibility – material-biology interactions, chemical/morphological/structural communication, mechanobiology, signaling and biological responses, immuno-engineering, calcification, coatings, corrosion and degradation of biomaterials and devices, biophysical regulation of cell functions Characterization, Synthesis, and Modification – new biomaterials, bioinspired and biomimetic approaches to biomaterials, exploiting structural hierarchy and architectural control, combinatorial strategies for biomaterials discovery, genetic biomaterials design, synthetic biology, new composite systems, bionics, polymer synthesis Controlled Release and Delivery Systems – biomaterial-based drug and gene delivery, bio-responsive delivery of regulatory molecules, pharmaceutical engineering Healthcare Advances – clinical translation, regulatory issues, patient safety, emerging trends Imaging and Diagnostics – imaging agents and probes, theranostics, biosensors, monitoring Manufacturing and Technology – 3D printing, inks, organ-on-a-chip, bioreactor/perfusion systems, microdevices, BioMEMS, optics and electronics interfaces with biomaterials, systems integration Modeling and Informatics Tools – scaling methods to guide biomaterial design, predictive algorithms for structure-function, biomechanics, integrating bioinformatics with biomaterials discovery, metabolomics in the context of biomaterials Tissue Engineering and Regenerative Medicine – basic and applied studies, cell therapies, scaffolds, vascularization, bioartificial organs, transplantation and functionality, cellular agriculture