Hierarchical F-actin microstructures and multi-passage viscoelasticity evolution in living cancer cells under varying glucose environment

IF 3.8 2区工程技术 Q1 ENGINEERING, MECHANICAL

Acta Mechanica Sinica Pub Date : 2024-07-26 DOI:10.1007/s10409-024-24243-x

Zhongyu Li (, ), Zhichao Li (, ), Songyou Yao (, ), He Jiang (, ), Xiaoyue Zhang (, ), Yue Zheng (, ), Wenpeng Zhu (, )

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Abstract

F-actin microstructures dominate cellular viscoelasticity and have been used to identify the migration and malignance of living cancer cells. Diabetic cancer patients suffer from increased metastasis and tumor recurrence. However, the long-term evolution and correlation of F-actin microstructures and viscoelasticity distribution are still poorly understood in living cancer cells under varying glucose environment. Herein, by using atomic force microscopy with amplitude modulation-frequency modulation and nanoindentation mode, we characterized the hierarchical F-actin microstructures and the multi-passage viscoelasticity evolution in living Huh-7 cancer cells transferred from high to low glucose level. The highly oriented stress fibers connected by thinner fiber networks were observed in high glucose environment. The circumferential actin networks composed by straight segment-like fibers and the randomly distributed actin fragments connected by ultrathin crosslinking fibers were observed in low glucose environment. The viscoelasticity within the nucleus and the cytoplasm of living Huh-7 cancer cells showed long-term fluctuations over tens of passages after switching glucose environments. The viscoelasticity of cytoplasm was more responsive to the change of glucose environments than nucleus, which was due to the reorganization of F-actin microstructures. Our work provides the microstructural and nanomechanical understanding on the migration and proliferation of living cancer cells under varying glucose environment.

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不同葡萄糖环境下活癌细胞中的分层 F-肌动蛋白微结构和多通道粘弹性演变

F 肌动蛋白微结构主导着细胞的粘弹性，已被用于识别活体癌细胞的迁移和恶性程度。糖尿病癌症患者的转移和肿瘤复发率都在增加。然而，人们对不同葡萄糖环境下活癌细胞的 F-肌动蛋白微结构和粘弹性分布的长期演变和相关性仍知之甚少。在此，我们利用原子力显微镜的振幅调制-频率调制和纳米压痕模式，表征了从高葡萄糖水平转移到低葡萄糖水平的活体Huh-7癌细胞中分层的F-肌动蛋白微结构和多通道粘弹性演变。在高糖环境中观察到了由较细纤维网络连接的高取向应力纤维。在低糖环境中，观察到由直线段状纤维组成的周向肌动蛋白网络和由超细交联纤维连接的随机分布的肌动蛋白片段。活的 Huh-7 癌细胞在转换葡萄糖环境后，细胞核和细胞质内的粘弹性在数十个传代中呈现长期波动。与细胞核相比，细胞质的粘弹性对葡萄糖环境变化的反应更灵敏，这是由于F-肌动蛋白微结构重组所致。我们的研究从微观结构和纳米力学角度揭示了活体癌细胞在不同葡萄糖环境下的迁移和增殖过程。

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来源期刊

Acta Mechanica Sinica 物理-工程：机械

CiteScore

5.60

自引率

20.00%

发文量

1807

审稿时长

4 months

期刊介绍： Acta Mechanica Sinica, sponsored by the Chinese Society of Theoretical and Applied Mechanics, promotes scientific exchanges and collaboration among Chinese scientists in China and abroad. It features high quality, original papers in all aspects of mechanics and mechanical sciences. Not only does the journal explore the classical subdivisions of theoretical and applied mechanics such as solid and fluid mechanics, it also explores recently emerging areas such as biomechanics and nanomechanics. In addition, the journal investigates analytical, computational, and experimental progresses in all areas of mechanics. Lastly, it encourages research in interdisciplinary subjects, serving as a bridge between mechanics and other branches of engineering and the sciences. In addition to research papers, Acta Mechanica Sinica publishes reviews, notes, experimental techniques, scientific events, and other special topics of interest. Related subjects » Classical Continuum Physics - Computational Intelligence and Complexity - Mechanics