A sandwich-type electrochemical sensor based on RGO-CeO2-Au nanoparticles and double aptamers for ultrasensitive detection of glypican-3

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2024-10-19 DOI:10.1007/s00604-024-06704-7

Jintao Liang, Jianlu Liang, Xinkai Xiao, Fei Guo, Yulian Zhan, Xueqing Zhou, Zhide Zhou, Guiyin Li

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Abstract

A sandwich-type electrochemical aptasensor for ultrasensitive detection of glypican-3 (GPC3) was constructed using GPC3 aptamer (GPC3_Apt) labelled reduced graphene oxide-cerium oxide-gold nanoparticles (RGO-CeO₂-Au NPs) as the signal probe and the same GPC3_Apt as the capture probe. The electrochemical redox properties of CeO₂ (Ce³⁺/Ce⁴⁺) in the RGO-CeO₂-Au NPs indicate the electrochemical signals. When the target GPC3 was present, an “aptamer-protein-aptamer” sandwich structure was formed on the sensing interface due to the specific binding between the protein and aptamers, resulting in an increased electrochemical redox signal detected by differential pulse voltammetry (DPV) technique. Under optimal conditions, the established aptasensor exhibited a logarithmic linear relationship between the current response and GPC3 concentration in the range 0.001–100.0 ng/mL, with a minimum detection limit of 0.74 pg/mL. Using the spike-recovery tests for measurement of the human serum samples, the recovery was from 99.26 to 114.01%, and the RSD range was 3.04 to 5.34%. Furthermore, the sandwich-type electrochemical aptasensor exhibited excellent performance characteristics such as good stability, high specificity, and high sensitivity, demonstrating effective detection of GPC3 in human serum samples and can be used as a clinical detection tool for GPC3.

Graphical Abstract

Abstract Image

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基于 RGO-CeO2-Au 纳米粒子和双适配体的夹层式电化学传感器，用于超灵敏检测 glypican-3

以标记了还原氧化石墨烯-氧化铈-金纳米粒子（RGO-CeO2-Au NPs）的 GPC3 aptamer（GPC3Apt）为信号探针，以相同的 GPC3Apt 为捕获探针，构建了超灵敏检测 glypican-3 (GPC3) 的夹层式电化学适配体传感器。RGO-CeO2-Au NPs 中 CeO2 的电化学氧化还原特性（Ce3+/Ce4+）显示了电化学信号。当目标 GPC3 存在时，由于蛋白质与适配体之间的特异性结合，在传感界面上形成了 "适配体-蛋白质-适配体 "的夹层结构，从而导致通过差分脉冲伏安法（DPV）技术检测到的电化学氧化还原信号增加。在最佳条件下，所建立的适配体传感器在 0.001-100.0 纳克/毫升范围内的电流响应与 GPC3 浓度之间呈现对数线性关系，最低检测限为 0.74 皮克/毫升。利用加标回收试验测量人体血清样品，回收率为 99.26% 至 114.01%，RSD 范围为 3.04% 至 5.34%。此外，该夹心型电化学适配传感器还具有稳定性好、特异性强、灵敏度高等优良性能特点，可有效检测人血清样品中的GPC3，可作为GPC3的临床检测工具。图文摘要

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.