Assessing the effects of NapA gene overexpression on denitrification and denitrogenation in magnetospirillum gryphiswaldense MSR-1

IF 2.3 3区 生物学 Q3 MICROBIOLOGY
Jiawei Hu, Meijing Liu, Lu Li, Jinjing Hu, Cong Wang
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引用次数: 0

Abstract

Previous research on Magnetospirillum gryphiswaldense MSR-1 found that MSR-1 has a good denitrification nitrogen removal ability and specific application prospects in the sewage biological nitrogen removal field. Therefore, this study selected the essential denitrification gene NapA in MSR-1-wt for overexpression, and the overexpressed MSR-1-NapA was successfully constructed. Q-PCR amplification experiment and AGAR gel electrophoresis experiment proved that the relative transcription level of the NapA gene was increased by more than four times, and the denitrification ability of MSR-1-wt and MSR-1-NapA was further determined by enzyme activity experiment, denitrification experiment, and flow cytometry. The results showed that overexpression of the NapA gene increased nitrate reductase activity in MSR-1-NapA by more than four times. In the solution with a nitrate concentration of 118.33 ± 3.23 mgN/L, the denitrification efficiency of MSR-1-NapA was superior to that of MSR-1-wt, significantly enhancing both the denitrification and nitrogen removal capacities of MSR-1. This indicates its greater potential for biological nitrogen removal in wastewater treatment.

评估NapA基因过表达对磁螺旋体MSR-1中反硝化和反氮化的影响
以往对Magnetospirillum gryphiswaldense MSR-1的研究发现,MSR-1具有良好的反硝化脱氮能力,在污水生物脱氮领域具有广阔的应用前景。因此,本研究选择了 MSR-1-wt 中的重要反硝化基因 NapA 进行过表达,并成功构建了过表达的 MSR-1-NapA。Q-PCR扩增实验和AGAR凝胶电泳实验证明,NapA基因的相对转录水平提高了4倍以上,并通过酶活性实验、反硝化实验和流式细胞术进一步测定了MSR-1-wt和MSR-1-NapA的反硝化能力。结果表明,过表达 NapA 基因后,MSR-1-NapA 的硝酸还原酶活性提高了四倍以上。在硝酸盐浓度为 118.33 ± 3.23 mgN/L 的溶液中,MSR-1-NapA 的反硝化效率优于 MSR-1-wt,显著提高了 MSR-1 的反硝化能力和脱氮能力。这表明 MSR-1 在废水处理中具有更大的生物脱氮潜力。
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来源期刊
Archives of Microbiology
Archives of Microbiology 生物-微生物学
CiteScore
4.90
自引率
3.60%
发文量
601
审稿时长
3 months
期刊介绍: Research papers must make a significant and original contribution to microbiology and be of interest to a broad readership. The results of any experimental approach that meets these objectives are welcome, particularly biochemical, molecular genetic, physiological, and/or physical investigations into microbial cells and their interactions with their environments, including their eukaryotic hosts. Mini-reviews in areas of special topical interest and papers on medical microbiology, ecology and systematics, including description of novel taxa, are also published. Theoretical papers and those that report on the analysis or ''mining'' of data are acceptable in principle if new information, interpretations, or hypotheses emerge.
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