Isolation and identification of epiphytic Pichia kudriavzevii from loquat peels and investigation of its fermentation characteristics for liquor production

IF 2.3 3区 生物学 Q3 MICROBIOLOGY
Qingfang Xu, Wei Huang, Yanqin Li, Jian Cai, Xiu Gao, Xu Bai, Weiliang Liu, Lifang Zhang, Ling Zhu
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引用次数: 0

Abstract

During the process of fruit wine production, yeast plays a crucial role in influencing the taste, flavor, and overall quality of the wine. This study aims to enhance the flavor and quality of loquat wine by isolating strains of Pichia kudriavzevii (P. kudriavzevii) with desirable winemaking characteristics from loquat fruit fermentation broth. A total of 12 strains of P. kudriavzevii were isolated and subjected to morphological and molecular biological identification. Their fermentation performance, ethanol production, ester production, hydrogen sulfide production, killer activity, and tolerance were evaluated. The results revealed that strains Q-2, Q-9, Q-10, Q-12, Q-20, and Q-42 exhibited robust growth and strong tolerance under conditions of 40 °C temperature, 12% ethanol concentration, 350 g/L glucose concentration, and pH 2.8. Strain Q-42 demonstrated the strongest gas production capacity, killer activity, and good ester and ethanol production. As a highly active fermentation strain with excellent wine making characteristics, P. kudriavzevii Q-42 provides a valuable yeast resource for the industrial production of loquat wine and offers technical support for improving the overall quality of loquat wine.

从枇杷皮中分离和鉴定附生的 Pichia kudriavzevii,并研究其用于白酒生产的发酵特性
在果酒酿造过程中,酵母对葡萄酒的口感、风味和整体质量起着至关重要的影响作用。本研究旨在从枇杷果实发酵液中分离出具有理想酿酒特性的 Pichia kudriavzevii(P. kudriavzevii)菌株,从而提高枇杷酒的风味和质量。共分离出 12 株 P. kudriavzevii,并对其进行了形态学和分子生物学鉴定。对它们的发酵性能、乙醇产量、酯产量、硫化氢产量、杀菌活性和耐受性进行了评估。结果显示,菌株 Q-2、Q-9、Q-10、Q-12、Q-20 和 Q-42 在温度为 40 °C、乙醇浓度为 12%、葡萄糖浓度为 350 克/升和 pH 值为 2.8 的条件下生长旺盛,耐受性强。菌株 Q-42 表现出最强的产气能力、杀菌活性以及良好的酯和乙醇产量。P. kudriavzevii Q-42 是一种高活性发酵菌株,具有优良的酿酒特性,为枇杷酒的工业化生产提供了宝贵的酵母资源,为提高枇杷酒的整体质量提供了技术支持。
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来源期刊
Archives of Microbiology
Archives of Microbiology 生物-微生物学
CiteScore
4.90
自引率
3.60%
发文量
601
审稿时长
3 months
期刊介绍: Research papers must make a significant and original contribution to microbiology and be of interest to a broad readership. The results of any experimental approach that meets these objectives are welcome, particularly biochemical, molecular genetic, physiological, and/or physical investigations into microbial cells and their interactions with their environments, including their eukaryotic hosts. Mini-reviews in areas of special topical interest and papers on medical microbiology, ecology and systematics, including description of novel taxa, are also published. Theoretical papers and those that report on the analysis or ''mining'' of data are acceptable in principle if new information, interpretations, or hypotheses emerge.
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