Production of docosahexaenoic acid through enzymatic hydrolysis of Omega-3 rich oil

IF 6.5 1区化学 Q2 CHEMISTRY, PHYSICAL

Journal of Catalysis Pub Date : 2024-10-18 DOI:10.1016/j.jcat.2024.115797

Ernestina García-Quinto , Raquel Aranda-Cañada , Jose M. Guisan , Gloria Fernandez-Lorente

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Abstract

Docosahexaenoic acid (DHA), an essential omega-3 fatty acid, is crucial for the normal development and function of the brain. Its production can be achieved through the partial hydrolysis of DHA-rich triglycerides catalyzed by lipases, particularly from fish oils. In this work, the characterization of anchovy oil capsules revealed that they offer a pure and concentrated source of DHA, with more than 90 % of the oil in the form of triglycerides. Therefore, the hydrolysis reaction was studied with the aim of releasing 100 % of the DHA present in this oil, using different immobilized lipases with maximum enzyme loading on the hydrophobic support Immobeads-C18. The study’s results revealed that our immobilization strategy through hydrophobic adsorption improved the catalytic properties of activity and selectivity of the TLL lipase compared to other biocatalysts described in the literature. Additionally, complete hydrolysis of the oil was achieved in just 24 h with the NS40-C18 derivative, which could be reused up to 6 cycles without loss of activity.

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通过酶水解富含 Omega-3 的油脂生产二十二碳六烯酸

二十二碳六烯酸（DHA）是一种必需的欧米伽-3 脂肪酸，对大脑的正常发育和功能至关重要。它可以通过脂肪酶催化富含 DHA 的甘油三酯部分水解而产生，特别是从鱼油中提取。在这项研究中，对凤尾鱼油胶囊的特性分析表明，它们是一种纯净而浓缩的 DHA 来源，其中 90% 以上的油以甘油三酯的形式存在。因此，我们研究了水解反应，目的是在疏水性支持物 Immobeads-C18 上使用不同的固定化脂肪酶，以最大的酶载量释放出这种油中 100% 的 DHA。研究结果表明，与文献中描述的其他生物催化剂相比，我们通过疏水吸附的固定化策略提高了 TLL 脂肪酶的催化活性和选择性。此外，使用 NS40-C18 衍生物仅需 24 小时就能完全水解油脂，而且该衍生物可重复使用长达 6 个周期而不会丧失活性。

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来源期刊

Journal of Catalysis 工程技术-工程：化工

CiteScore

12.30

自引率

5.50%

发文量

447

审稿时长

31 days

期刊介绍： The Journal of Catalysis publishes scholarly articles on both heterogeneous and homogeneous catalysis, covering a wide range of chemical transformations. These include various types of catalysis, such as those mediated by photons, plasmons, and electrons. The focus of the studies is to understand the relationship between catalytic function and the underlying chemical properties of surfaces and metal complexes. The articles in the journal offer innovative concepts and explore the synthesis and kinetics of inorganic solids and homogeneous complexes. Furthermore, they discuss spectroscopic techniques for characterizing catalysts, investigate the interaction of probes and reacting species with catalysts, and employ theoretical methods. The research presented in the journal should have direct relevance to the field of catalytic processes, addressing either fundamental aspects or applications of catalysis.