Tailored controllable bifunctional fluorescent-magnetic nanobeads for new generation fast-response immunosensor with dual-signal amplification

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical Pub Date : 2024-10-18 DOI:10.1016/j.snb.2024.136804

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Abstract

Immunosensors based on the fluorescence-linked immunosorbent assay (FLISA) platform are now widely used; however, the long-term response and lower sensitivity limited their development in clinical applications. In this study, we first tailored bifunctional magnetic nanoparticle (MNP)-quantum dot (QD) nanobeads (MQBs) with rapid magnetic response and strong fluorescence intensity for the FLISA platform to construct a fast-response immunosensor. Under optimal conditions, we constructed a dual-signal amplification immunosensor based on an MQB-driven FLISA platform for a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antigen. We achieved a 29-fold increase in fluorescence intensity over the QD nanobeads (SiO₂/QD/SiO₂, QBs). Compared with the conventional FLISA platform-based QBs, the sensitivity of the developed immunosensor increased 63-fold to 7.50 pg/mL in half the detection time of common FLISA (only 30 min). More important, the test results of this dual-signal amplification immunosensor showed good specificity and accuracy. This novel MQB magnetic-driven FLISA platform with high throughput, as a proof of concept, effectively improved sensitivity and shortened reaction time. As a result, it is expected to have significant potential in the field of point-of-care testing.

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量身定制的可控双功能荧光磁性纳米吸附剂，用于新一代双信号放大快速反应免疫传感器

基于荧光联免疫吸附测定（FLISA）平台的免疫传感器目前已得到广泛应用，但其长期响应和较低的灵敏度限制了其在临床应用中的发展。在本研究中，我们首先为 FLISA 平台定制了具有快速磁响应和强荧光强度的双功能磁性纳米颗粒（MNP）-量子点（QD）纳米吸附珠（MQBs），构建了一种快速响应免疫传感器。在最佳条件下，我们构建了一种基于 MQB 驱动 FLISA 平台的双信号放大免疫传感器，用于检测严重急性呼吸系统综合征冠状病毒 2（SARS-CoV-2）抗原。与 QD 纳米吸附珠（SiO2/QD/SiO2，QBs）相比，我们的荧光强度提高了 29 倍。与基于传统 FLISA 平台的 QBs 相比，所开发的免疫传感器的灵敏度提高了 63 倍，达到 7.50 pg/mL，而检测时间仅为普通 FLISA 的一半（30 分钟）。更重要的是，这种双信号放大免疫传感器的检测结果显示出良好的特异性和准确性。作为概念验证，这种新型 MQB 磁驱动 FLISA 高通量平台有效提高了灵敏度，缩短了反应时间。因此，它有望在护理点检测领域大显身手。

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来源期刊

Sensors and Actuators B: Chemical 工程技术-电化学

CiteScore

14.60

自引率

11.90%

发文量

1776

审稿时长

3.2 months

期刊介绍： Sensors & Actuators, B: Chemical is an international journal focused on the research and development of chemical transducers. It covers chemical sensors and biosensors, chemical actuators, and analytical microsystems. The journal is interdisciplinary, aiming to publish original works showcasing substantial advancements beyond the current state of the art in these fields, with practical applicability to solving meaningful analytical problems. Review articles are accepted by invitation from an Editor of the journal.