Molecular Identification and Fungicide Sensitivity of Ceratobasidium sp. (AG-A) Isolates Causing Stem Canker on Potato in Mexico

IF 1.1 4区农林科学 Q3 PLANT SCIENCES

Journal of Phytopathology Pub Date : 2024-10-18 DOI:10.1111/jph.13412

Rosalía López-Corrales, Sami J. Michereff, Kamila Câmara Correia, Raymundo S. García-Estrada, Edgar H. Nieto-López, Guadalupe A. Mora-Romero, Juan M. Tovar-Pedraza

引用次数: 0

Abstract

In 2022, potato plants exhibiting symptoms of stem canker were observed in commercial fields in northern Sinaloa, Mexico. Morphological characterisation of two isolates allowed the identification of Ceratobasidium sp., whereas molecular analysis using sequences of the ITS region and the rpb2 gene distinguished the isolates within the Anastomosis Group A (AG-A). Both isolates induced symptoms of stem canker on inoculated potato plants and exhibited median EC₅₀ values of 19.46, 5.20, 62.71 and 0.33 μg mL⁻¹ for the fungicides thiophanate methyl, pyraclostrobin, prochloraz and penflufen, respectively. However, the two isolates differed by > 65-fold in their EC₅₀ value relative to pyraclostrobin.

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墨西哥引起马铃薯茎腐烂病的 Ceratobasidium sp. (AG-A) 分离物的分子鉴定和杀菌剂敏感性

2022 年，在墨西哥锡那罗亚州北部的商品田中观察到马铃薯植株出现茎腐病症状。通过对两株分离物的形态特征进行分析，确定其为 Ceratobasidium sp.，而利用 ITS 区域和 rpb2 基因序列进行的分子分析则将这两株分离物区分为吻合组 A（AG-A）。两种分离物都会诱导接种马铃薯植株出现茎腐病症状，对杀菌剂甲基硫菌灵、吡唑醚菌酯、丙氯禾草灵和五氟苯的 EC50 中值分别为 19.46、5.20、62.71 和 0.33 μg mL-1。不过，这两种分离物对吡唑醚菌酯的半数致死浓度值相差 65 倍。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Phytopathology 生物-植物科学

CiteScore

2.90

自引率

0.00%

发文量

审稿时长

4-8 weeks

期刊介绍： Journal of Phytopathology publishes original and review articles on all scientific aspects of applied phytopathology in agricultural and horticultural crops. Preference is given to contributions improving our understanding of the biotic and abiotic determinants of plant diseases, including epidemics and damage potential, as a basis for innovative disease management, modelling and forecasting. This includes practical aspects and the development of methods for disease diagnosis as well as infection bioassays. Studies at the population, organism, physiological, biochemical and molecular genetic level are welcome. The journal scope comprises the pathology and epidemiology of plant diseases caused by microbial pathogens, viruses and nematodes. Accepted papers should advance our conceptual knowledge of plant diseases, rather than presenting descriptive or screening data unrelated to phytopathological mechanisms or functions. Results from unrepeated experimental conditions or data with no or inappropriate statistical processing will not be considered. Authors are encouraged to look at past issues to ensure adherence to the standards of the journal.