Artificially obtained humic-like substances from chicken manure and symbionts in in vitro and in situ improvement of oil degradation in soil

IF 1.827 Q2 Earth and Planetary Sciences

Arabian Journal of Geosciences Pub Date : 2024-10-18 DOI:10.1007/s12517-024-12105-0

Olga Senko, Nikolay Stepanov, Olga Maslova, Marina Gladchenko, Sergey Gaydamaka, Aysel Aslanli, Elena Efremenko

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Abstract

Alkaline hydrolysate of chicken manure (HCM) containing artificially formed humic-like substances (HLSs) was investigated in combination with specially loaded cells (bacteria and microalgae) for oil degradation in soil. After 7 days in the laboratory experiment, the oil biodegradation efficiency in the polluted soil (50 g oil/kg soil) was 5–78% in 11 soil samples depending on the soil characteristics and used combination of additives. Higher level of oil-degradation corresponded to soil with high initial concentration of humic substances (HSs) (85–107 g/kg of dry matter). The best result in bioremediation (degradation of 82% of oil for 7 days) was achieved in experiment under field conditions with the similar initial oil pollution in the soil, when the HCM was used as additive (up to 220 mg HLSs/kg dry soil) in combination with an artificial symbionts composed of Rhodococcus erythropolis and Chlorella vulgaris cells. The introduction of HCM into oil-polluted soil with the mentioned cells led to an increase in all controlled enzymatic activities (peroxidase, dehydrogenase, phosphatase, protease, amylase, and urease) in the soil. The observed effects testified to activation of cell metabolic processes in the soil. As a result, the introduction of HCM in combination with artificial symbionts into the soil increased the rate of oil biodegradation under environmental conditions up to 1.8 times.

Graphical Abstract

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从鸡粪和共生菌中人工获得的类腐殖质在体外和原位改善土壤中的石油降解作用

研究了含有人工形成的腐殖样物质（HLSs）的碱性鸡粪水解物（HCM）与特殊负载细胞（细菌和微藻）在土壤中降解石油的组合。经过 7 天的实验室实验，根据土壤特性和所使用添加剂的组合，11 个土壤样本中受污染土壤（50 克油/千克土壤）的石油生物降解效率为 5-78%。腐殖质（HSs）初始浓度较高（85-107 克/千克干物质）的土壤的油降解率较高。在土壤中初始油污染相似的实地条件下进行的实验中，将 HCM 作为添加剂（高达 220 毫克腐殖质/千克干土）与由红球菌和小球藻细胞组成的人工共生体结合使用，取得了最佳的生物修复效果（7 天内降解了 82% 的油）。将 HCM 与上述细胞一起引入油污染土壤后，土壤中所有受控酶活性（过氧化物酶、脱氢酶、磷酸酶、蛋白酶、淀粉酶和脲酶）都有所增加。观察到的效果证明，土壤中的细胞代谢过程被激活了。因此，将 HCM 与人工共生体结合引入土壤后，石油在环境条件下的生物降解速度提高了 1.8 倍。

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来源期刊

Arabian Journal of Geosciences GEOSCIENCES, MULTIDISCIPLINARY-

自引率

0.00%

发文量

1587

审稿时长

6.7 months

期刊介绍： The Arabian Journal of Geosciences is the official journal of the Saudi Society for Geosciences and publishes peer-reviewed original and review articles on the entire range of Earth Science themes, focused on, but not limited to, those that have regional significance to the Middle East and the Euro-Mediterranean Zone. Key topics therefore include; geology, hydrogeology, earth system science, petroleum sciences, geophysics, seismology and crustal structures, tectonics, sedimentology, palaeontology, metamorphic and igneous petrology, natural hazards, environmental sciences and sustainable development, geoarchaeology, geomorphology, paleo-environment studies, oceanography, atmospheric sciences, GIS and remote sensing, geodesy, mineralogy, volcanology, geochemistry and metallogenesis.