Sperm penetration at the maturing metaphase I stage can trigger oocyte activation in a mouse model

IF 3.7 2区医学 Q1 OBSTETRICS & GYNECOLOGY

Reproductive biomedicine online Pub Date : 2024-06-21 DOI:10.1016/j.rbmo.2024.104329

Ching-Chien Chang , Min Peng , Li-Kuang Tsai , Chia-Chun Chang , Chia-Jung Li , Chia-Kuan Wu , Chin-Cheng Chien , Jie Xu , Zsolt Peter Nagy , Chi-Hong Liu , Chung-Hao Lu , Li-Ying Sung

{"title":"Sperm penetration at the maturing metaphase I stage can trigger oocyte activation in a mouse model","authors":"Ching-Chien Chang , Min Peng , Li-Kuang Tsai , Chia-Chun Chang , Chia-Jung Li , Chia-Kuan Wu , Chin-Cheng Chien , Jie Xu , Zsolt Peter Nagy , Chi-Hong Liu , Chung-Hao Lu , Li-Ying Sung","doi":"10.1016/j.rbmo.2024.104329","DOIUrl":null,"url":null,"abstract":"<div><h3>Research question</h3><div>Can spermatozoa penetrate maturing metaphase I (MI) oocytes, and render subsequent development following conventional IVF in a mouse model?</div></div><div><h3>Design</h3><div>ICR mice were used in this study. Metaphase II (MII) cumulus–oocyte complexes (COC) harvested 15 h after injection of human chorionic gonadotrophin (HCG) were used for IVF as the control group (Group 1). In the treatment group (Group 2), maturing MI COC harvested 7 h after HCG injection were used for IVF. Fertilization, pronuclear formation, cleavage, blastocyst formation, DNA methylation status, chromosome number and live birth rates were used to evaluate the developmental dynamics and competency of maturing MI oocytes following conventional IVF.</div></div><div><h3>Results</h3><div>Maturing MI COC were fertilized using conventional IVF, and sperm penetration at MI–telophase I triggered oocyte activation. Most embryos resulting from fertilized MI oocytes developed to blastocyst stage during preimplantation development, albeit a substantial proportion of them were triploids due to the absence of the second meiotic division. Some of the embryos derived from fertilization of maturing oocytes were able to implant and gave rise to full-term development.</div></div><div><h3>Conclusion</h3><div>Maturing MI COC from follicles before ovulation could be used for mouse IVF, and fertilized MI oocytes had high potential for development. Healthy offspring can be generated from maturing MI COC following conventional IVF. MI COC may represent a valuable source of ‘usable’ biomaterial in assisted reproduction. However, many embryos derived from MI COC via IVF have abnormal chromosome numbers in the mouse model. The implications of these findings for human IVF remain to be investigated.</div></div>","PeriodicalId":21134,"journal":{"name":"Reproductive biomedicine online","volume":null,"pages":null},"PeriodicalIF":3.7000,"publicationDate":"2024-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Reproductive biomedicine online","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1472648324005182","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"OBSTETRICS & GYNECOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Research question

Can spermatozoa penetrate maturing metaphase I (MI) oocytes, and render subsequent development following conventional IVF in a mouse model?

Design

ICR mice were used in this study. Metaphase II (MII) cumulus–oocyte complexes (COC) harvested 15 h after injection of human chorionic gonadotrophin (HCG) were used for IVF as the control group (Group 1). In the treatment group (Group 2), maturing MI COC harvested 7 h after HCG injection were used for IVF. Fertilization, pronuclear formation, cleavage, blastocyst formation, DNA methylation status, chromosome number and live birth rates were used to evaluate the developmental dynamics and competency of maturing MI oocytes following conventional IVF.

Results

Maturing MI COC were fertilized using conventional IVF, and sperm penetration at MI–telophase I triggered oocyte activation. Most embryos resulting from fertilized MI oocytes developed to blastocyst stage during preimplantation development, albeit a substantial proportion of them were triploids due to the absence of the second meiotic division. Some of the embryos derived from fertilization of maturing oocytes were able to implant and gave rise to full-term development.

Conclusion

Maturing MI COC from follicles before ovulation could be used for mouse IVF, and fertilized MI oocytes had high potential for development. Healthy offspring can be generated from maturing MI COC following conventional IVF. MI COC may represent a valuable source of ‘usable’ biomaterial in assisted reproduction. However, many embryos derived from MI COC via IVF have abnormal chromosome numbers in the mouse model. The implications of these findings for human IVF remain to be investigated.

查看原文本刊更多论文

在小鼠模型中，精子在成熟分裂期 I 阶段的穿透可触发卵母细胞活化

研究问题在小鼠模型中，精子能否穿透成熟的分裂期 I（MI）卵母细胞，并使其在常规体外受精后继续发育？对照组（第1组）在注射人绒毛膜促性腺激素（HCG）15小时后收获的成熟分裂期II（MII）卵母细胞复合体（COC）用于体外受精。治疗组（第 2 组）采用注射 HCG 7 小时后收获的成熟 MI COC 进行体外受精。通过受精、原核形成、裂解、囊胚形成、DNA甲基化状态、染色体数目和活产率来评估常规体外受精后成熟 MI 卵母细胞的发育动态和能力。受精 MI 卵母细胞产生的大多数胚胎在植入前发育过程中发育到囊胚期，但由于缺乏第二次减数分裂，其中相当一部分是三倍体。结论从排卵前的卵泡中提取成熟的 MI COC 可用于小鼠体外受精，受精的 MI 卵母细胞具有很高的发育潜力。在常规体外受精过程中，成熟的MI COC可产生健康的后代。MI COC可能是辅助生殖中 "可用 "生物材料的宝贵来源。然而，在小鼠模型中，许多通过体外受精从 MI COC 获得的胚胎染色体数目异常。这些发现对人类试管婴儿的影响仍有待研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Reproductive biomedicine online 医学-妇产科学

CiteScore

7.20

自引率

7.50%

发文量

391

审稿时长

50 days

期刊介绍： Reproductive BioMedicine Online covers the formation, growth and differentiation of the human embryo. It is intended to bring to public attention new research on biological and clinical research on human reproduction and the human embryo including relevant studies on animals. It is published by a group of scientists and clinicians working in these fields of study. Its audience comprises researchers, clinicians, practitioners, academics and patients. Context: The period of human embryonic growth covered is between the formation of the primordial germ cells in the fetus until mid-pregnancy. High quality research on lower animals is included if it helps to clarify the human situation. Studies progressing to birth and later are published if they have a direct bearing on events in the earlier stages of pregnancy.