Drivers of denitrification and nitrification in a dryland agroecosystem: The role of abiotic and biotic factors

IF 4.8 2区 农林科学 Q1 SOIL SCIENCE
Zhou Fang , Zhenling Peng , Yali Zhang , Jiayao Han , Jianbin Pan , Qi Zhang , Lin Mao , Guoxi Shi , Yongjun Liu , Huyuan Feng , Xavier Le Roux
{"title":"Drivers of denitrification and nitrification in a dryland agroecosystem: The role of abiotic and biotic factors","authors":"Zhou Fang ,&nbsp;Zhenling Peng ,&nbsp;Yali Zhang ,&nbsp;Jiayao Han ,&nbsp;Jianbin Pan ,&nbsp;Qi Zhang ,&nbsp;Lin Mao ,&nbsp;Guoxi Shi ,&nbsp;Yongjun Liu ,&nbsp;Huyuan Feng ,&nbsp;Xavier Le Roux","doi":"10.1016/j.apsoil.2024.105691","DOIUrl":null,"url":null,"abstract":"<div><div>Agricultural practices such as tillage and fertilization impact soil nitrogen (N) cycling processes, but how they alter the coupling between the activity, abundance and diversity of N-cycling microbes remains to be understood. Here, we used a fifteen-year trial in a dryland agroecosystem on the Loess Plateau of China (two tillage regimes crossed with six fertilization treatments) to understand how (de)nitrification potentials are determined by soil abiotic conditions and the abundances and compositions of the (de)nitrifier communities. We measured the abundances of bacterial (AOB) and archaeal (AOA) ammonia oxidizers and <em>nirK</em>- and <em>nirS</em>-nitrite reducers, their community compositions, potential nitrification (PNA) and denitrification (PDA), and soil abiotic conditions. PNA and PDA across the 12 treatments were positively correlated to AOB abundance and <em>nirS</em> abundance, respectively. Co-occurrence network analysis revealed the presence of dominant ecological modules of (de)nitrifiers sensitive to agricultural treatments, and more complex network under no-tilled than tilled conditions as well as under multiple fertilizers than unfertilized conditions. Path analysis and random forest analysis both showed that PNA was explained by AOB abundances and the relative abundance of one module of (de)nitrifiers driven by soil ammonium concentration, while PDA was most related to soil organic carbon concentration, pH and to a lesser extent <em>nirS</em> abundance. These findings demonstrate that, in agricultural soils, the potential of denitrification –a facultative activity for denitrifiers– is mainly predicted by abiotic conditions, while the potential of nitrification –an obligate activity for nitrifiers– is determined by biotic variables, here AOB abundances and a particular cluster of microbial populations.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"204 ","pages":"Article 105691"},"PeriodicalIF":4.8000,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied Soil Ecology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0929139324004220","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"SOIL SCIENCE","Score":null,"Total":0}
引用次数: 0

Abstract

Agricultural practices such as tillage and fertilization impact soil nitrogen (N) cycling processes, but how they alter the coupling between the activity, abundance and diversity of N-cycling microbes remains to be understood. Here, we used a fifteen-year trial in a dryland agroecosystem on the Loess Plateau of China (two tillage regimes crossed with six fertilization treatments) to understand how (de)nitrification potentials are determined by soil abiotic conditions and the abundances and compositions of the (de)nitrifier communities. We measured the abundances of bacterial (AOB) and archaeal (AOA) ammonia oxidizers and nirK- and nirS-nitrite reducers, their community compositions, potential nitrification (PNA) and denitrification (PDA), and soil abiotic conditions. PNA and PDA across the 12 treatments were positively correlated to AOB abundance and nirS abundance, respectively. Co-occurrence network analysis revealed the presence of dominant ecological modules of (de)nitrifiers sensitive to agricultural treatments, and more complex network under no-tilled than tilled conditions as well as under multiple fertilizers than unfertilized conditions. Path analysis and random forest analysis both showed that PNA was explained by AOB abundances and the relative abundance of one module of (de)nitrifiers driven by soil ammonium concentration, while PDA was most related to soil organic carbon concentration, pH and to a lesser extent nirS abundance. These findings demonstrate that, in agricultural soils, the potential of denitrification –a facultative activity for denitrifiers– is mainly predicted by abiotic conditions, while the potential of nitrification –an obligate activity for nitrifiers– is determined by biotic variables, here AOB abundances and a particular cluster of microbial populations.
旱地农业生态系统中脱氮和硝化的驱动因素:非生物和生物因素的作用
耕作和施肥等农业实践会影响土壤氮(N)循环过程,但它们如何改变氮循环微生物的活性、丰度和多样性之间的耦合关系仍有待了解。在此,我们在中国黄土高原的一个旱地农业生态系统中进行了为期 15 年的试验(两种耕作制度与六种施肥处理交叉进行),以了解(脱)硝化潜力是如何由土壤非生物条件以及(脱)硝化物群落的丰度和组成决定的。我们测量了细菌(AOB)和古菌(AOA)氨氧化剂以及 nirK- 和 nirS-亚硝酸盐还原剂的丰度、群落组成、潜在硝化(PNA)和反硝化(PDA)以及土壤非生物条件。12 种处理的 PNA 和 PDA 分别与 AOB 丰度和 nirS 丰度呈正相关。共现网络分析显示,存在对农业处理敏感的(脱)硝因子的主导生态模块,而且在免耕条件下比耕作条件下以及在施用多种肥料条件下比未施肥条件下的网络更复杂。路径分析和随机森林分析均表明,PNA 是由土壤铵浓度驱动的 AOB 丰度和(脱)硝因子模块的相对丰度解释的,而 PDA 与土壤有机碳浓度、pH 值的关系最大,与 nirS 丰度的关系较小。这些研究结果表明,在农业土壤中,反硝化潜力(反硝化菌的兼性活动)主要由非生物条件预测,而硝化潜力(硝化菌的强制性活动)则由生物变量决定,这里的生物变量指的是AOB丰度和特定的微生物种群群集。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Applied Soil Ecology
Applied Soil Ecology 农林科学-土壤科学
CiteScore
9.70
自引率
4.20%
发文量
363
审稿时长
5.3 months
期刊介绍: Applied Soil Ecology addresses the role of soil organisms and their interactions in relation to: sustainability and productivity, nutrient cycling and other soil processes, the maintenance of soil functions, the impact of human activities on soil ecosystems and bio(techno)logical control of soil-inhabiting pests, diseases and weeds.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信