Enhancement of structural properties of 3D-printed plant-based meat analogs by TGase/laccase

IF 5.3 2区农林科学 Q1 ENGINEERING, CHEMICAL

Journal of Food Engineering Pub Date : 2024-10-09 DOI:10.1016/j.jfoodeng.2024.112352

Zhi Cheng , Yue Qiu , Ishtiaq Ahmad , Yuting Pang , Aodong Yue , Zhangxi Chen , Gaopeng Zhang , Yuting Ding , Fei Lyu

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引用次数: 0

Abstract

As we all know, achieving the desirable texture and fibrous structure of 3D-printed meat analogs has been challenging. Therefore, this study aimed to investigate the effect of transglutaminase (TGase) or laccase on printability, texture, and structure of 3D-printed soy protein isolate (SPI) -wheat gluten (WG) -insoluble dietary fiber (IDF) plant-based meat analogs (PBMA). Results showed that TGase significantly improved the hardness, gumminess, and chewiness of PBMA (p < 0.05), whereas laccase exhibited an opposite trend. The tensile strength and elongation at break were increased by TGase or laccase, with laccase exhibiting better effect (9.37 ± 0.25 kPa, 63.16 ± 3.18%). The interaction of laccase and IDF resulted in the maximum value of apparent viscosity and disulfide bond content, which hindered the movement of printed materials through the printing nozzle, and potentially led to PBMA incomplete-structure. Synergistic effect of TGase and IDF has been shown to enhance the content of hydrogen, hydrophobic, and disulfide bonds. This combination readily induced an increase in α-helix and β-sheet formations, promoting a more orderly development of the protein structure, consequently, the internal architecture became more uniform and densely packed. These results indicated that TGase treatment combined with IDF might be a novel and promising strategy to enhance the texture and structure of PBMA.

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利用 TGase/laccase 增强三维打印植物肉类类似物的结构特性

众所周知，实现 3D 打印肉类类似物的理想质地和纤维结构一直是一项挑战。因此，本研究旨在探讨转谷氨酰胺酶（TGase）或漆酶对三维打印大豆分离蛋白（SPI）-小麦面筋（WG）-不溶性膳食纤维（IDF）植物基肉类类似物（PBMA）的可打印性、质地和结构的影响。结果表明，TGase 能明显改善 PBMA 的硬度、胶粘性和咀嚼性（p < 0.05），而漆酶则表现出相反的趋势。TGase 或漆酶都能提高拉伸强度和断裂伸长率，其中漆酶的效果更好（9.37 ± 0.25 kPa，63.16 ± 3.18%）。漆酶和 IDF 的相互作用导致表观粘度和二硫键含量达到最大值，从而阻碍了印刷材料在印刷喷嘴中的移动，并可能导致 PBMA 结构不完整。事实证明，TGase 和 IDF 的协同作用可提高氢键、疏水键和二硫键的含量。这种组合很容易诱导α-螺旋和β-片的形成，促进蛋白质结构更有序地发展，从而使内部结构变得更加均匀和致密。这些结果表明，TGase 处理与 IDF 结合可能是增强 PBMA 质地和结构的一种新颖而有前途的策略。

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来源期刊

Journal of Food Engineering 工程技术-工程：化工

CiteScore

11.80

自引率

5.50%

发文量

275

审稿时长

24 days

期刊介绍： The journal publishes original research and review papers on any subject at the interface between food and engineering, particularly those of relevance to industry, including: Engineering properties of foods, food physics and physical chemistry; processing, measurement, control, packaging, storage and distribution; engineering aspects of the design and production of novel foods and of food service and catering; design and operation of food processes, plant and equipment; economics of food engineering, including the economics of alternative processes. Accounts of food engineering achievements are of particular value.