{"title":"Pinus radiata seedlings rhizobiome shifts in response to foliar and root phosphite application","authors":"Frederico Leitão , Glória Pinto , Isabel Henriques","doi":"10.1016/j.ejsobi.2024.103688","DOIUrl":null,"url":null,"abstract":"<div><div>Soil health is an emerging concern in agriculture and is dependent on the microbial communities in the rhizosphere (rhizobiome). Phosphite-based products are used as bio-stimulants and/or fungicides. However, there is a lack of studies evaluating the impact of these products in nurseries, especially at the level of the rhizobiome. This work aims to assess the impact of phosphite (Phi) application on the rhizobiome of <em>Pinus radiata</em> seedlings. Two application modes (foliar and irrigation) were compared in an experimental setup with control treatments. Gas exchange parameters were evaluated to assess plant physiological performance. Bacterial rhizobiome analysis was performed using next generation sequencing targeting the 16S rRNA gene. Results showed that Phi application did not significantly affect plant photosynthetic performance. However, Phi irrigation led to a significant decrease in rhizobiome richness and diversity compared to control. Beta diversity analysis confirmed distinct microbial communities in the irrigated group. At the genus level, several acidophilic taxa, including <em>Burkholderia</em> and <em>Aciditerrimonas</em>, were significantly enriched in phosphite-irrigated samples, while others like <em>Mucilaginibacter</em> were reduced. The study reveals that Phi application, especially through irrigation, alters the structure of the rhizobiome in pine seedlings, leading to a decrease in richness and bacterial diversity. These findings highlight the importance of understanding the effects of commercial products, such as phosphite. This understanding is crucial to ensure sustainable plant growth and maintain soil health.</div></div>","PeriodicalId":12057,"journal":{"name":"European Journal of Soil Biology","volume":"123 ","pages":"Article 103688"},"PeriodicalIF":3.7000,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"European Journal of Soil Biology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1164556324000943","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ECOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Soil health is an emerging concern in agriculture and is dependent on the microbial communities in the rhizosphere (rhizobiome). Phosphite-based products are used as bio-stimulants and/or fungicides. However, there is a lack of studies evaluating the impact of these products in nurseries, especially at the level of the rhizobiome. This work aims to assess the impact of phosphite (Phi) application on the rhizobiome of Pinus radiata seedlings. Two application modes (foliar and irrigation) were compared in an experimental setup with control treatments. Gas exchange parameters were evaluated to assess plant physiological performance. Bacterial rhizobiome analysis was performed using next generation sequencing targeting the 16S rRNA gene. Results showed that Phi application did not significantly affect plant photosynthetic performance. However, Phi irrigation led to a significant decrease in rhizobiome richness and diversity compared to control. Beta diversity analysis confirmed distinct microbial communities in the irrigated group. At the genus level, several acidophilic taxa, including Burkholderia and Aciditerrimonas, were significantly enriched in phosphite-irrigated samples, while others like Mucilaginibacter were reduced. The study reveals that Phi application, especially through irrigation, alters the structure of the rhizobiome in pine seedlings, leading to a decrease in richness and bacterial diversity. These findings highlight the importance of understanding the effects of commercial products, such as phosphite. This understanding is crucial to ensure sustainable plant growth and maintain soil health.
期刊介绍:
The European Journal of Soil Biology covers all aspects of soil biology which deal with microbial and faunal ecology and activity in soils, as well as natural ecosystems or biomes connected to ecological interests: biodiversity, biological conservation, adaptation, impact of global changes on soil biodiversity and ecosystem functioning and effects and fate of pollutants as influenced by soil organisms. Different levels in ecosystem structure are taken into account: individuals, populations, communities and ecosystems themselves. At each level, different disciplinary approaches are welcomed: molecular biology, genetics, ecophysiology, ecology, biogeography and landscape ecology.