PaLAR3 genotype variability for enhanced resistance against Heterobasidion parviporum in Norway spruce: Insights into allelic frequencies, disease response, and the role of mycoviruses

IF 3.7 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biological Control Pub Date : 2024-10-12 DOI:10.1016/j.biocontrol.2024.105633

Muhammad Kashif, Eeva Terhonen, Leena Hamberg, Tuula Piri, Matti Haapanen, Sirkku Pöykkö, Eeva J. Vainio, Jarkko Hantula

{"title":"PaLAR3 genotype variability for enhanced resistance against Heterobasidion parviporum in Norway spruce: Insights into allelic frequencies, disease response, and the role of mycoviruses","authors":"Muhammad Kashif, Eeva Terhonen, Leena Hamberg, Tuula Piri, Matti Haapanen, Sirkku Pöykkö, Eeva J. Vainio, Jarkko Hantula","doi":"10.1016/j.biocontrol.2024.105633","DOIUrl":null,"url":null,"abstract":"<div><div><em>Heterobasidion parviporum</em> is a destructive forest pathogen causing root and stem rot of Norway spruce (<em>Picea abies</em>) in northern hemisphere. Previous research has suggested that a gene form of PaLAR3 that encodes for the leucoanthocyanidin reductase enzyme, may increase resistance to <em>H. parviporum</em> in Norway spruce, whether heterozygous or homozygous. Our experiment was initially focused on testing this assertion with materials from the Finnish Norway spruce breeding program, comprising 52 full-sib families. Additionally, we investigated the influence of mycoviruses on the growth of <em>H. parviporum</em>. The frequencies of the three PaLAR3 genotypes AA, AB, and BB were 64%, 30%, and 6%, respectively. We found no significant differences in the necrotic response to <em>H. parviporum</em> between the homozygous (BB) and heterozygous (AB) genotypes vs. the homozygous (AA) genotype across the two fungal strains tested, suggesting the gene is rather ineffective as a marker for overall <em>H. parviporum</em> resistance. The two <em>H. parviporum</em> strains used in the study showed significantly different necrosis responses from the control. Mycovirus infection in <em>H. parviporum</em> strains led to a significant reduction in lesion width in phloem and sapwood. Furthermore, the growth of the fungus was notably restricted when hyphae hosted mycoviruses, indicating a high potential of mycoviruses to reduce fungal growth and necrosis development.</div></div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"198 ","pages":"Article 105633"},"PeriodicalIF":3.7000,"publicationDate":"2024-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biological Control","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1049964424001981","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Heterobasidion parviporum is a destructive forest pathogen causing root and stem rot of Norway spruce (Picea abies) in northern hemisphere. Previous research has suggested that a gene form of PaLAR3 that encodes for the leucoanthocyanidin reductase enzyme, may increase resistance to H. parviporum in Norway spruce, whether heterozygous or homozygous. Our experiment was initially focused on testing this assertion with materials from the Finnish Norway spruce breeding program, comprising 52 full-sib families. Additionally, we investigated the influence of mycoviruses on the growth of H. parviporum. The frequencies of the three PaLAR3 genotypes AA, AB, and BB were 64%, 30%, and 6%, respectively. We found no significant differences in the necrotic response to H. parviporum between the homozygous (BB) and heterozygous (AB) genotypes vs. the homozygous (AA) genotype across the two fungal strains tested, suggesting the gene is rather ineffective as a marker for overall H. parviporum resistance. The two H. parviporum strains used in the study showed significantly different necrosis responses from the control. Mycovirus infection in H. parviporum strains led to a significant reduction in lesion width in phloem and sapwood. Furthermore, the growth of the fungus was notably restricted when hyphae hosted mycoviruses, indicating a high potential of mycoviruses to reduce fungal growth and necrosis development.

查看原文本刊更多论文

PaLAR3基因型变异增强了挪威云杉对Heterobasidion parviporum的抗性：对等位基因频率、疾病反应和霉菌病毒作用的见解

Heterobasidion parviporum 是一种破坏性森林病原体，会导致北半球的挪威云杉（Picea abies）根腐病和茎腐病。以前的研究表明，编码白花青素还原酶的 PaLAR3 基因形式可能会增加挪威云杉（无论是杂合还是同源）对 H. parviporum 的抗性。我们的实验最初主要是利用芬兰挪威云杉育种计划中的材料（包括52个全同源系）来验证这一论断。此外，我们还研究了霉菌病毒对副云杉生长的影响。三种PaLAR3基因型AA、AB和BB的频率分别为64%、30%和6%。我们发现，在所测试的两种真菌菌株中，同源基因型（BB）和杂合子基因型（AB）与同源基因型（AA）对副猪痢疾杆菌的坏死反应没有明显差异，这表明该基因作为副猪痢疾杆菌总体抗性的标记并不有效。研究中使用的两种 H. parviporum 菌株的坏死反应与对照有显著差异。真菌病毒感染 H. parviporum 菌株会导致韧皮部和边材的病变宽度显著减小。此外，当菌丝寄生真菌病毒时，真菌的生长明显受到限制，这表明真菌病毒在减少真菌生长和坏死发展方面具有很大的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Biological Control 生物-昆虫学

CiteScore

7.40

自引率

7.10%

发文量

220

审稿时长

63 days

期刊介绍： Biological control is an environmentally sound and effective means of reducing or mitigating pests and pest effects through the use of natural enemies. The aim of Biological Control is to promote this science and technology through publication of original research articles and reviews of research and theory. The journal devotes a section to reports on biotechnologies dealing with the elucidation and use of genes or gene products for the enhancement of biological control agents. The journal encompasses biological control of viral, microbial, nematode, insect, mite, weed, and vertebrate pests in agriculture, aquatic, forest, natural resource, stored product, and urban environments. Biological control of arthropod pests of human and domestic animals is also included. Ecological, molecular, and biotechnological approaches to the understanding of biological control are welcome.