Learning from the desert legume tree, Prosopis cineraria to develop stress-tolerant crops

IF 4.5 2区 生物学 Q2 ENVIRONMENTAL SCIENCES
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Abstract

Plants inhabiting adverse growth conditions compete against stresses by the endogenous regulatory elements viz., promoters and terminators at the ‘right time’ (time-of-stress-act), ‘right place’ (tissue-of-act), and ‘right expression’ (time-of-transcription). Heat stress at the reproductive stage impedes pollen viability and stigma receptiveness, affecting the seed set. Prosopis cineraria, the dominant desert-inhabiting legume tree, is heat- and drought-tolerant. The distribution of heat shock elements in a heat-inducible promoter determines the magnitudes of target gene expression in different tissues/organs. Relative expression of P. cineraria heat shock protein 18.2 (PcHsp18.2) in alternate months of 2021 displayed the highest expression in summer. The flowers collected in June, the hottest month (47 °C) of 2021, exhibited a high expression of PcHsp18.2. The germination of the pollen collected was 80 %, and the trees eventually set seeds. Comprehensive analysis of the promoter (pPcHSP18.2) and terminator (tPchsp18.2) of PcHsp18.2 by expressing the gusA in tobacco exhibited the highest expression under heat stress as similar to the expression of PcHsp18.2 in environmental samples. Ectopic expression of gusA under PcHsp18.2 promoter and terminator resulted in an increased seed set due to the viability of pollen and stigma under heat stress. The gusA expression under PcHsp18.2 promoter and terminator was high-fold in anther compared to the Lat52 and g10 (endogenous genes under its promoter and terminator) genes under heat stress. The expression of genes under strong and balanced endogenous inducible promoter and terminator combinations, as in the desert-growing P. cineraria in transgenic plants, enables the development of resilient crops.
向沙漠豆科植物--红花楹学习,开发抗逆作物
在不利的生长条件下,植物通过内源调控元件,即启动子和终止子,在 "正确的时间"(胁迫作用时间)、"正确的地点"(作用组织)和 "正确的表达"(转录时间)与胁迫进行竞争。生殖阶段的热胁迫会阻碍花粉的活力和柱头的接受能力,影响种子的结实。在沙漠中栖息的主要豆科树种红花楹(Prosopis cineraria)具有耐热和耐旱性。热诱导启动子中热休克元件的分布决定了目标基因在不同组织/器官中的表达量。P. cineraria 热休克蛋白 18.2(PcHsp18.2)在 2021 年隔月的相对表达量在夏季最高。在 2021 年最热(47 °C)的 6 月份采集的花朵中,PcHsp18.2 的表达量较高。采集到的花粉发芽率为 80%,树木最终结出了种子。通过在烟草中表达gusA,对PcHsp18.2的启动子(pPcHSP18.2)和终止子(tPchsp18.2)进行了综合分析,结果表明在热胁迫下PcHsp18.2的表达量最高,与环境样本中PcHsp18.2的表达量相似。在 PcHsp18.2 启动子和终止子下异位表达 gusA,可提高花粉和柱头在热胁迫下的活力,从而提高结实率。在热胁迫下,PcHsp18.2 启动子和终止子下的 gusA 在花药中的表达量是 Lat52 和 g10(其启动子和终止子下的内源基因)基因表达量的数倍。在转基因植物中,如在沙漠生长的 P. cineraria 中,基因在强且平衡的内源诱导型启动子和终止子组合下表达,可培育出抗逆性强的作物。
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来源期刊
Environmental and Experimental Botany
Environmental and Experimental Botany 环境科学-环境科学
CiteScore
9.30
自引率
5.30%
发文量
342
审稿时长
26 days
期刊介绍: Environmental and Experimental Botany (EEB) publishes research papers on the physical, chemical, biological, molecular mechanisms and processes involved in the responses of plants to their environment. In addition to research papers, the journal includes review articles. Submission is in agreement with the Editors-in-Chief. The Journal also publishes special issues which are built by invited guest editors and are related to the main themes of EEB. The areas covered by the Journal include: (1) Responses of plants to heavy metals and pollutants (2) Plant/water interactions (salinity, drought, flooding) (3) Responses of plants to radiations ranging from UV-B to infrared (4) Plant/atmosphere relations (ozone, CO2 , temperature) (5) Global change impacts on plant ecophysiology (6) Biotic interactions involving environmental factors.
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