Shahad K. Alsaiari, Behnaz Eshaghi, Bujie Du, Maria Kanelli, Gary Li, Xunhui Wu, Linzixuan Zhang, Mehr Chaddah, Alicia Lau, Xin Yang, Robert Langer, Ana Jaklenec
{"title":"CRISPR–Cas9 delivery strategies for the modulation of immune and non-immune cells","authors":"Shahad K. Alsaiari, Behnaz Eshaghi, Bujie Du, Maria Kanelli, Gary Li, Xunhui Wu, Linzixuan Zhang, Mehr Chaddah, Alicia Lau, Xin Yang, Robert Langer, Ana Jaklenec","doi":"10.1038/s41578-024-00725-7","DOIUrl":null,"url":null,"abstract":"<p>CRISPR–Cas9 genome editing technology is a promising tool for genetically engineering immune cells and modulating immune systems. Although ex vivo genome editing of immune cells has reached clinical trials, in vivo application is still restricted by the instability and inefficient delivery of CRISPR–Cas9 components to immune cells through circulation. In this Review, we summarize ex vivo and in vivo strategies to deliver CRISPR–Cas9 components to both non-immune and immune cells. We review the progress made in non-immune cells because it offers insights that can be applied to advancing research in immune cells. We also discuss principles and challenges of immune system modulation using CRISPR–Cas9 genome editing technology.</p>","PeriodicalId":19081,"journal":{"name":"Nature Reviews Materials","volume":null,"pages":null},"PeriodicalIF":79.8000,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nature Reviews Materials","FirstCategoryId":"88","ListUrlMain":"https://doi.org/10.1038/s41578-024-00725-7","RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MATERIALS SCIENCE, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
CRISPR–Cas9 genome editing technology is a promising tool for genetically engineering immune cells and modulating immune systems. Although ex vivo genome editing of immune cells has reached clinical trials, in vivo application is still restricted by the instability and inefficient delivery of CRISPR–Cas9 components to immune cells through circulation. In this Review, we summarize ex vivo and in vivo strategies to deliver CRISPR–Cas9 components to both non-immune and immune cells. We review the progress made in non-immune cells because it offers insights that can be applied to advancing research in immune cells. We also discuss principles and challenges of immune system modulation using CRISPR–Cas9 genome editing technology.
期刊介绍：
Nature Reviews Materials is an online-only journal that is published weekly. It covers a wide range of scientific disciplines within materials science. The journal includes Reviews, Perspectives, and Comments.
Nature Reviews Materials focuses on various aspects of materials science, including the making, measuring, modelling, and manufacturing of materials. It examines the entire process of materials science, from laboratory discovery to the development of functional devices.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
