Simultaneous determination of somatic cell count and total plate count in raw milk based on ATP bioluminescence assay

IF 5.7 2区 化学 Q1 CHEMISTRY, ANALYTICAL
Longrui Yang , Xiaoyun Sun , Jiaci Chen , Juan Zhang , Xiaoyu Li , Song Qu , Kai Wu , Fengchun Huang , Ailiang Chen
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Abstract

The somatic cell count (SCC) and total plate count (TPC) are essential quality indicators for raw milk. Traditional detection methods require separate measurements and rely on complex, large-scale instruments or cultivation techniques, which are both time-consuming and laborious. To address these challenges, this study developed a novel method for the simultaneous detection of SCC and TPC in the same raw milk sample using the ATP bioluminescence assay. This method utilizes oxy-ethylated iso-nonyl phenol (Neonol-10) and cetyltrimethylammonium bromide (CTAB) to selectively lyse somatic cells and microorganisms, respectively. This technique is straightforward to operate and can be completed within 2.5 h, with detection ranges of 1 × 10⁴ to 3 × 10⁶ cells/mL for SCC and 1 × 10⁵ to 5 × 10⁷ CFU/mL for TPC. Importantly, this technique meets the requirements of detection standards in China, European Union, Canada, United States, etc. For SCC or TPC in raw milk. Overall, this innovative approach does not rely on expensive equipment or facilities and the stepwise reagent addition procedure can be easily developed into an automated high-throughput system for rapid on-site testing of SCC and TPC in raw milk.

Abstract Image

Abstract Image

基于 ATP 生物发光检测法同时测定生乳中的体细胞数和菌落总数
体细胞计数(SCC)和总平板计数(TPC)是生鲜乳的重要质量指标。传统的检测方法需要分别测量,并依赖于复杂的大型仪器或培养技术,既费时又费力。为了应对这些挑战,本研究开发了一种新方法,利用 ATP 生物发光检测法同时检测同一生乳样品中的 SCC 和 TPC。该方法利用氧乙基异壬基酚(Neonol-10)和十六烷基三甲基溴化铵(CTAB)分别选择性地裂解体细胞和微生物。该技术操作简单,可在 2.5 小时内完成,对 SCC 的检测范围为 1×10⁴ 至 3×10⁶ cells/mL,对 TPC 的检测范围为 1×10⁵ 至 5×10⁷ CFU/mL。重要的是,该技术符合中国、欧盟、加拿大、美国等国对生乳中 SCC 或 TPC 检测标准的要求。总之,这种创新方法不依赖于昂贵的设备或设施,逐步添加试剂的程序可以很容易地开发成一个自动化的高通量系统,用于现场快速检测生乳中的 SCC 和 TPC。
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来源期刊
Analytica Chimica Acta
Analytica Chimica Acta 化学-分析化学
CiteScore
10.40
自引率
6.50%
发文量
1081
审稿时长
38 days
期刊介绍: Analytica Chimica Acta has an open access mirror journal Analytica Chimica Acta: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. Analytica Chimica Acta provides a forum for the rapid publication of original research, and critical, comprehensive reviews dealing with all aspects of fundamental and applied modern analytical chemistry. The journal welcomes the submission of research papers which report studies concerning the development of new and significant analytical methodologies. In determining the suitability of submitted articles for publication, particular scrutiny will be placed on the degree of novelty and impact of the research and the extent to which it adds to the existing body of knowledge in analytical chemistry.
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