Aerobic bacteria induced biomineralization: effects of nutrient and calcium content on the nanostructure and chemical composition of simulated cement mixture

IF 10.8 1区工程技术 Q1 CONSTRUCTION & BUILDING TECHNOLOGY

Cement & concrete composites Pub Date : 2024-10-16 DOI:10.1016/j.cemconcomp.2024.105801

Linzhen Tan , Jiacheng Zhang , Jing Xu , Binling Chen , Baosen Mi , Yongqi Wei , Wu Yao

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引用次数: 0

Abstract

During the past two decades, aerobic bacteria induced biomineralization has gained popularity for autonomous sealing of cracks in concrete structures due to its environmentally friendly characteristics of carbon retention. However, the mechanism of the biomineralization induced by aerobic bacteria for concrete crack sealing is still unclear due to the complex chemistry of cement matrix. Also, as the main nutrient for bacterial growth, the effect of yeast extract (YE) on biomineralization should be properly evaluated. For the first time, this study investigates the effects of YE and calcium content on the development of nanostructure and chemical composition of cement matrix during the biomineralization process induced by aerobic bacteria Bacillus cohnii. The effects of calcium content were realized by constructing a simulated cement mixture consisting of calcium hydroxide and synthesised C-S-H with different C/S ratios. The effects of YE content were evaluated by the addition of different amounts of YE into simulated cement mixture with different C/S ratios. Results suggest that 10g/l of YE can cause a high pH value of 10 and an unsuccessful growth of bacteria in simulated cement mixture with C/S ratios 0.6 and 0.8. Upon increasing the YE from 10 g/l to 20 g/l, the pH reduced from 10 to 9 and the bacteria was successfully grown. Higher amounts of bicarbonate ions can cause an enhanced decalcification of synthesised C-S-H rather than calcium hydroxide. The aerobic bacteria Bacillus cohnii showed superior performance on calcium absorption and calcite precipitation, being a promising way for practical concrete crack sealing.

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好氧菌诱导的生物矿化：养分和钙含量对模拟水泥混合物纳米结构和化学成分的影响

在过去的二十年里，好氧菌诱导的生物矿化因其保碳的环境友好特性，在混凝土结构裂缝的自主密封方面得到了普及。然而，由于水泥基质的化学性质复杂，好氧菌诱导生物矿化用于混凝土裂缝密封的机理仍不清楚。此外，作为细菌生长的主要营养物质，酵母提取物（YE）对生物矿化的影响也应适当评估。本研究首次探讨了在好氧菌诱导的生物矿化过程中，酵母提取物和钙含量对水泥基质纳米结构的发展和化学成分的影响。钙含量的影响是通过构建由氢氧化钙和不同 C/S 比合成的 C-S-H 组成的模拟水泥混合物来实现的。通过在不同 C/S 比的模拟水泥混合物中添加不同量的 YE，评估了 YE 含量的影响。结果表明，在 C/S 比为 0.6 和 0.8 的模拟水泥混合物中，10 克/升的 YE 会导致 pH 值达到 10，细菌无法成功生长。当 YE 从 10 克/升增加到 20 克/升时，pH 值从 10 降到 9，细菌成功生长。碳酸氢根离子含量越高，合成的 C-S-H 脱钙效果越好，而不是氢氧化钙。好氧细菌枯草芽孢杆菌在钙吸收和方解石沉淀方面表现出色，是一种很有前途的混凝土裂缝密封方法。

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来源期刊

Cement & concrete composites 工程技术-材料科学：复合

CiteScore

18.70

自引率

11.40%

发文量

459

审稿时长

65 days

期刊介绍： Cement & concrete composites focuses on advancements in cement-concrete composite technology and the production, use, and performance of cement-based construction materials. It covers a wide range of materials, including fiber-reinforced composites, polymer composites, ferrocement, and those incorporating special aggregates or waste materials. Major themes include microstructure, material properties, testing, durability, mechanics, modeling, design, fabrication, and practical applications. The journal welcomes papers on structural behavior, field studies, repair and maintenance, serviceability, and sustainability. It aims to enhance understanding, provide a platform for unconventional materials, promote low-cost energy-saving materials, and bridge the gap between materials science, engineering, and construction. Special issues on emerging topics are also published to encourage collaboration between materials scientists, engineers, designers, and fabricators.