Pelargonic acid’s interaction with the auxin transporter PIN1: A potential mechanism behind its phytotoxic effects on plant metabolism

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science Pub Date : 2024-10-10 DOI:10.1016/j.plantsci.2024.112278

David López-González , Marta Muñoz Usero , José M. Hermida-Ramón , Sara Álvarez-Rodríguez , Fabrizio Araniti , Marta Teijeira , Mercedes Verdeguer , Adela M. Sánchez-Moreiras

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引用次数: 0

Abstract

Pelargonic acid (PA) is a saturated fatty acid commonly found in several organisms, that is known for its phytotoxic effect and its use as bioherbicide for sustainable weed management. Although PA is already commercialised as bioherbicide, its molecular targets and mode of action is unknown according to the Herbicide Resistance Action Committee. Therefore, the aim of this work was focusing on the way this natural active substance impacts the plant metabolism of the model species Arabidopsis thaliana. PA caused increase of secondary and adventitious roots, as well as torsion, loss of gravitropism and phytotoxic effects. Moreover, PA altered the cellular arrangement and the PIN proteins activity. Computational simulations revealed that the intermolecular interactions between PA and the polar auxin transporter protein PIN1 are very similar to those established between the natural auxin IAA and PIN1. However, under intracellular conditions, the PA-PIN1 binding is more energetically stable than the IAA-PIN1. These results suggest that PA could act as an auxin-mimics bioherbicide. The exogenous application of PA would be responsible for the alterations observed both at structural and ultrastructural levels, which would be caused by the alteration on the transport of auxins into the plant, inducing root inhibition and ultimately total stop of root growth.

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壬二酸与植物生长素转运体 PIN1 的相互作用：壬二酸对植物新陈代谢产生植物毒性作用的潜在机制

壬二酸（Pelargonic acid，PA）是一种饱和脂肪酸，通常存在于多种生物体内，因其植物毒性作用而闻名，可用作可持续杂草管理的生物除草剂。虽然 PA 已作为生物除草剂实现商业化，但除草剂抗性行动委员会对其分子靶标和作用模式尚不清楚。因此，这项工作的目的是重点研究这种天然活性物质对模式物种拟南芥（Arabidopsis thaliana）植物新陈代谢的影响。PA 会导致次生根和不定根的增加，以及扭转、失去引力和植物毒性效应。此外，PA 还改变了细胞排列和 PIN 蛋白的活性。计算模拟显示，PA 与极性植物生长素转运蛋白 PIN1 之间的分子间相互作用与天然植物生长素 IAA 与 PIN1 之间的相互作用非常相似。然而，在细胞内条件下，PA 与 PIN1 的结合比 IAA 与 PIN1 的结合在能量上更稳定。这些结果表明，PA 可作为一种模拟助剂的生物杀草剂。外源施用 PA 将导致在结构和超微结构水平上观察到的变化，这些变化将由改变植物体内的辅素运输引起，从而导致根抑制，最终完全停止根的生长。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Science 生物-生化与分子生物学

CiteScore

9.10

自引率

1.90%

发文量

322

审稿时长

33 days

期刊介绍： Plant Science will publish in the minimum of time, research manuscripts as well as commissioned reviews and commentaries recommended by its referees in all areas of experimental plant biology with emphasis in the broad areas of genomics, proteomics, biochemistry (including enzymology), physiology, cell biology, development, genetics, functional plant breeding, systems biology and the interaction of plants with the environment. Manuscripts for full consideration should be written concisely and essentially as a final report. The main criterion for publication is that the manuscript must contain original and significant insights that lead to a better understanding of fundamental plant biology. Papers centering on plant cell culture should be of interest to a wide audience and methods employed result in a substantial improvement over existing established techniques and approaches. Methods papers are welcome only when the technique(s) described is novel or provides a major advancement of established protocols.