Metabolic changes in response to food intake in somatostatin 1.1 deficient zebrafish

IF 1 Q4 GENETICS & HEREDITY

Gene Reports Pub Date : 2024-10-11 DOI:10.1016/j.genrep.2024.102062

Jie Chen , Huiming Yuan , Jing Gao , Lu Liu , Adelino V.M. Canario

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引用次数: 0

Abstract

Somatostatin is a multifunctional hormone with several genes in teleost fishes. A zebrafish CRISPR/Cas9 knockout of the somatostatin 1.1 (sst1.1) with persistent hyperglycaemia and hyperlipidaemia displayed reduced fecundity when fed brine shrimp ad libitum. Here, we investigated the effect of feeding brine shrimp one to three times a day on fecundity and liver transcriptomics of the sst1.1 mutant compared to their wild-type siblings to unravel molecular pathways associated with the phenotype. We find that the sst1.1 deficient zebrafish had high mortality when fed at the highest rate and that in both genotypes, growth and fecundity were proportional to food intake. Although glucose and cholesterol decreased substantially at the lowest level of feeding, they were still higher in the mutant than in the wild-type zebrafish. Furthermore, sst1.1 deficiency had a small but significant effect on the hepatic expression of protein, carbohydrate, and fatty acid biosynthesis genes, contributing to the mutant's diabetic phenotype.

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体节蛋白 1.1 缺乏症斑马鱼摄入食物后的代谢变化

体生长抑素是一种多功能激素，在远洋鱼类中有多个基因。斑马鱼CRISPR/Cas9基因敲除了体生长抑素1.1（sst1.1），这种斑马鱼具有持续的高血糖和高脂血症，在自由喂食卤虾时会降低繁殖力。在此，我们研究了与野生型同胞相比，每天喂食一至三次卤虾对 sst1.1 突变体的繁殖力和肝脏转录组学的影响，以揭示与该表型相关的分子通路。我们发现，sst1.1缺陷斑马鱼在摄食量最高的情况下死亡率很高，而且两种基因型的生长和繁殖力都与摄食量成正比。虽然葡萄糖和胆固醇在最低摄食量时大幅下降，但突变型斑马鱼的葡萄糖和胆固醇仍高于野生型斑马鱼。此外，sst1.1 的缺乏对肝脏中蛋白质、碳水化合物和脂肪酸生物合成基因的表达有微小但显著的影响，从而导致突变体的糖尿病表型。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Gene Reports Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.30

自引率

7.70%

发文量

246

审稿时长

49 days

期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.