Two-dimensional photonic crystal acetylcholinesterase hydrogel and organohydrogel sensors for efficient detection of organophosphorus compounds

Abstract

Sensors capable of detecting organophosphorus (OP) compounds have attracted the most attention owing to severe OP contamination worldwide. Despite many years of research, the developed OP sensors mainly focused on detecting water-soluble OPs in proper environments and the exploration of OP sensors suitable in resource-limited areas is extremely challenging. Here, a simple two-dimensional photonic crystal (2D PC) hydrogel featuring capabilities of effectively quantitative determination of OP compounds is facilely constructed by immobilizing the enzyme acetylcholinesterase (AChE) onto a bovine serum albumin (BSA) protein hydrogel. Owing to the specific interaction between AChE and OP compounds, the OP compounds are easily bound to the hydrogel, triggering volume phase transition and resulting in apparent Debye diffraction ring variations. The resulting hydrogel sensors show a limit of detection (LoD) of 2.23 nM for trichlorfon and 0.07 nM for diethyl methylphosphonate (DMPP), respectively. On the basis of the hydrogel, a responsive organohydrogel is facilely fabricated utilizing a solvent exchange strategy to meet the requirements of applications in harsh environments and detection of the non-water-soluble OP compounds. The organohydrogel sensors, however, demonstrated a LoD of 0.70 μM for trichlorfon and 4.46 μM for DMPP, respectively. This work provides new light on the development of next-generation stable, low-cost, and portable field sensing devices.