Specialization of Alcanivorax species in colonizing diverse plastics

IF 4.3 2区 生物学 Q2 MICROBIOLOGY
Valérie Mattelin, Astrid Rombouts, Josefien Van Landuyt, Alberto Scoma, Nico Boon
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Abstract

Recently, there has been increased attention to hydrocarbonoclastic bacteria in the plastisphere. One particular genus, Alcanivorax, is reported in the biodegradation of several polymers in the literature. In this study, we further explored the role of Alcanivorax in the early colonization of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBH), nylon 6/69, and a novel plastic B4PF01. Starting from enrichments of a one-year experiment with a maximum relative abundance of 58.8% of Alcanivorax, two parallel experiments were set up. One experiment followed growth and activity during the first 21 days of plastic incubations, and the other followed the same parameters on the different material fractions of the plastics, such as leachables and pure polymer. For all plastic types, the highest microbial growth was associated with the total plastics compared to the other material fractions. A relative abundance of 62.7% of Alcanivorax in the nylon 6/69-enriched community was observed. This, combined with data on activity, suggests that nylon 6/69 is potentially degraded by this genus. Two isolates were obtained, closely related to A. borkumensis SK2 and Alcanivorax sp. DG881. The activity and growth of the isolates as axenic cultures resemble their abundance in the community. In conclusion, this study contributes to the knowledge of the role of Alcanivorax in plastic-enriched communities.

Abstract Image

Alcanivorax 物种在不同塑料中的定殖特化
最近,人们越来越关注质体中的碳氢细菌。文献中报道了一个特殊的属--Alcanivorax--在几种聚合物的生物降解过程中的作用。在本研究中,我们进一步探讨了 Alcanivorax 在聚(3-羟基丁酸-3-羟基己酸)(PHBH)、尼龙 6/69 和新型塑料 B4PF01 早期定殖中的作用。在为期一年的实验中,Alcanivorax 的最大相对丰度为 58.8%,从富集开始,我们建立了两个平行实验。一项实验跟踪塑料培养头 21 天的生长和活性,另一项实验跟踪塑料不同材料部分(如可浸出物和纯聚合物)的相同参数。在所有类型的塑料中,与其他材料部分相比,总塑料的微生物生长量最高。在尼龙 6/69 富集群落中,Alcanivorax 的相对丰度为 62.7%。结合活性数据,这表明尼龙 6/69 有可能被该属降解。获得了两个与 A. borkumensis SK2 和 Alcanivorax sp.作为轴向培养物,这些分离物的活性和生长与它们在群落中的数量相似。总之,本研究有助于了解 Alcanivorax 在富塑群落中的作用。
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来源期刊
Environmental microbiology
Environmental microbiology 环境科学-微生物学
CiteScore
9.90
自引率
3.90%
发文量
427
审稿时长
2.3 months
期刊介绍: Environmental Microbiology provides a high profile vehicle for publication of the most innovative, original and rigorous research in the field. The scope of the Journal encompasses the diversity of current research on microbial processes in the environment, microbial communities, interactions and evolution and includes, but is not limited to, the following: the structure, activities and communal behaviour of microbial communities microbial community genetics and evolutionary processes microbial symbioses, microbial interactions and interactions with plants, animals and abiotic factors microbes in the tree of life, microbial diversification and evolution population biology and clonal structure microbial metabolic and structural diversity microbial physiology, growth and survival microbes and surfaces, adhesion and biofouling responses to environmental signals and stress factors modelling and theory development pollution microbiology extremophiles and life in extreme and unusual little-explored habitats element cycles and biogeochemical processes, primary and secondary production microbes in a changing world, microbially-influenced global changes evolution and diversity of archaeal and bacterial viruses new technological developments in microbial ecology and evolution, in particular for the study of activities of microbial communities, non-culturable microorganisms and emerging pathogens
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