Green-synthesized carbon dots from ginger: Multifunctional agents against oral pathogens with biocompatibility in human gingival fibroblast cells

IF 5.4 Q1 PLANT SCIENCES

Current Plant Biology Pub Date : 2024-10-09 DOI:10.1016/j.cpb.2024.100392

Shaeesta Khaleelahmed Bhavikatti , Siti Lailatul Akmar Zainuddin , Rosmaliza Binti Ramli , Sameer J. Nadaf , Padma B. Dandge , Masidd Khalate , Mohmed Isaqali Karobari

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引用次数: 0

Abstract

Persistent antibiotic use in treating oral infections often leads to drug resistance in pathogenic bacteria, notably impacting conditions like periodontitis. Addressing this challenge, the study pioneers the use of carbon dots (CDs) synthesized from ginger rhizomes (Zingiber officinale) as a novel biocompatible material. CDs were synthesized via the hydrothermal method, emphasizing a green approach, and comprehensively characterized for their optical properties and structural uniformity. The synthesized CDs showed a zeta potential of −24.9 mV, confirming the formation of stable and well-dispersed particles. Dynamic Light Scattering (DLS) confirmed an average particle size of 2.9 nm, thus validating the formation of CDs. Biomedical assessments demonstrated that the synthesized CDs were non-cytotoxic to human gingival fibroblast cell lines, with effective free radical scavenging activity and high total antioxidant capacity, as indicated by their IC50 values. CDs also exhibited moderate inhibition of protein denaturation compared to the standard. Moreover, they showed significant inhibitory effects against bacterial strains (Pseudomonas aeruginosa, Lactobacillus acidophilus, Escherichia coli, Staphylococcus aureus) and fungal strains (Aspergillus niger, Candida albicans) at minimal concentrations. Notably, CDs inhibited the growth of periodontal pathogens including Aggregatibacter actinomycetemcomitans, Tannerella forsythia, Porphyromonas gingivalis, and Prevotella intermedia. These findings underscore the potential of CDs as multifunctional agents possessing anti-inflammatory, antifungal, antioxidant, and antibacterial properties. Remarkably, they offer a promising alternative to conventional antibiotics, potentially revolutionizing oral healthcare. Their proven biocompatibility and potent bioactivity underscore their innovative potential in biomedical research. Future studies should further assess their efficacy in vivo to fully harness their clinical potential.

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生姜绿色合成碳点：在人牙龈成纤维细胞中具有生物相容性的抗口腔病原体多功能制剂

在治疗口腔感染时持续使用抗生素往往会导致病原菌产生耐药性，特别是对牙周炎等疾病造成影响。为了应对这一挑战，这项研究率先使用生姜根茎合成的碳点（CD）作为新型生物相容性材料。该研究采用水热法合成碳点，强调绿色环保，并对其光学特性和结构均匀性进行了全面表征。合成的 CD 的 zeta 电位为 -24.9 mV，证明形成了稳定且分散良好的颗粒。动态光散射（DLS）证实其平均粒径为 2.9 nm，从而验证了光盘的形成。生物医学评估表明，合成的 CD 对人类牙龈成纤维细胞系无毒性，具有有效的自由基清除活性和较高的总抗氧化能力（如其 IC50 值所示）。与标准物质相比，CDs 还能适度抑制蛋白质变性。此外，在最低浓度下，它们对细菌菌株（铜绿假单胞菌、嗜酸乳杆菌、大肠杆菌、金黄色葡萄球菌）和真菌菌株（黑曲霉、白色念珠菌）有明显的抑制作用。值得注意的是，CD 可抑制牙周病原体的生长，包括放线菌、连翘丹那菌、牙龈卟啉单胞菌和中间前驱菌。这些发现凸显了 CD 作为多功能制剂的潜力，它具有抗炎、抗真菌、抗氧化和抗菌特性。值得注意的是，它们有望成为传统抗生素的替代品，为口腔保健带来革命性的变化。经证实的生物相容性和强大的生物活性凸显了它们在生物医学研究中的创新潜力。未来的研究应进一步评估其体内疗效，以充分发挥其临床潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current Plant Biology Agricultural and Biological Sciences-Plant Science

CiteScore

10.90

自引率

1.90%

发文量

审稿时长

50 days

期刊介绍： Current Plant Biology aims to acknowledge and encourage interdisciplinary research in fundamental plant sciences with scope to address crop improvement, biodiversity, nutrition and human health. It publishes review articles, original research papers, method papers and short articles in plant research fields, such as systems biology, cell biology, genetics, epigenetics, mathematical modeling, signal transduction, plant-microbe interactions, synthetic biology, developmental biology, biochemistry, molecular biology, physiology, biotechnologies, bioinformatics and plant genomic resources.