LINC01322 may serve as a potential diagnostic marker for advanced stage tumors in renal cell carcinoma patients eligible for total nephrectomy

IF 2.3 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemistry and Biophysics Reports Pub Date : 2024-10-13 DOI:10.1016/j.bbrep.2024.101843

Amirhosein Maharati , Negin Taghehchian , Fatemeh Taghavinia , Alireza Golshan , Azadeh Aarabi , Mohammad Reza Abbaszadegan , Meysam Moghbeli

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引用次数: 0

Abstract

Background

Renal cell carcinoma (RCC) is a common urological cancer globally and shows a favorable prognosis in early stages of the tumor progression. Due to the poor prognosis for metastatic RCC patients, it is crucial to explore the molecular biology of RCC progression to establish efficient diagnostic and therapeutic markers for these patients. Long non-coding RNAs (lncRNAs) have critical roles in regulation of tumor cell proliferation, migration, and apoptosis during RCC progression. For the first time in the present study, we assessed the LINC01322 RNA expression levels in RCC patients to introduce that as a potential tumor marker among these patients.

Methods

we visualized LINC01322 expression data using the online tool Gene Expression Profiling Interactive Analysis (GEPIA2) across different cancers and normal tissues. Fifty fresh samples of RCC tumor tissues and their adjacent normal margins were collected to analyze the RNA expression of LINC01322 and its association with the clinicopathological features of RCC patients. The SYBR green method was used in real-time PCR to measure the LINC01322 RNA expression levels in RCC patients.

Results

Based on in-silico analysis, we hypothesized that LINC01322 could be involved in RCC progression by interacting with VHL, thereby influencing the tumor microenvironment. There were significant increased levels of LINC01322 RNA expressions in advanced stage compared with primary stage tumors that were located in left kidney (p = 0.048). Left kidney that were undergone the total nephrectomy had significant higher levels of LINC01322 RNA expressions compared with tumors in right kidney (p = 0.045). There was a direct correlation between the levels of LINC01322 RNA expression and RCC tumor size.

Conclusions

considering the substantial increase in LINC01322 RNA expression in advanced stage RCC tumors that are candidates for total nephrectomy; it could be suggested as a potential diagnostic indicator for high-risk patients. In-silico analysis also revealed that LINC01322 could be involved in regulation of tumor microenvironment during RCC progression by interacting with VHL. However, further investigations are needed to validate the potential link between LINC01322 and VHL during RCC progression. Evaluating the serum LINC01322 RNA levels in RCC patients is also necessary to use that as a diagnostic marker in clinical settings.

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LINC01322 可作为符合全肾切除术条件的肾细胞癌患者晚期肿瘤的潜在诊断标志物

背景肾细胞癌（RCC）是全球常见的泌尿系统癌症，在肿瘤进展的早期预后良好。由于转移性 RCC 患者预后较差，因此探索 RCC 进展的分子生物学至关重要，以便为这些患者建立有效的诊断和治疗标记。长非编码 RNA（lncRNA）在 RCC 进展过程中对肿瘤细胞的增殖、迁移和凋亡起着关键的调控作用。在本研究中，我们首次评估了LINC01322 RNA在RCC患者中的表达水平，并将其作为一种潜在的肿瘤标志物引入这些患者中。我们收集了 50 份新鲜的 RCC 肿瘤组织及其邻近的正常边缘组织样本，以分析 LINC01322 的 RNA 表达及其与 RCC 患者临床病理特征的关联。结果基于体内分析，我们推测LINC01322可能通过与VHL相互作用参与RCC进展，从而影响肿瘤微环境。与原发期肿瘤相比，位于左肾的晚期肿瘤的 LINC01322 RNA 表达水平明显升高（p = 0.048）。与右肾肿瘤相比，接受全肾切除术的左肾肿瘤的 LINC01322 RNA 表达水平明显更高（p = 0.045）。LINC01322的RNA表达水平与RCC肿瘤的大小直接相关。体内分析还显示，LINC01322 可能通过与 VHL 相互作用，在 RCC 进展过程中参与肿瘤微环境的调控。然而，要验证LINC01322与VHL在RCC进展过程中的潜在联系还需要进一步的研究。评估RCC患者血清中的LINC01322 RNA水平对于将其作为临床诊断标志物也很有必要。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biochemistry and Biophysics Reports Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

4.60

自引率

0.00%

发文量

191

审稿时长

59 days

期刊介绍： Open access, online only, peer-reviewed international journal in the Life Sciences, established in 2014 Biochemistry and Biophysics Reports (BB Reports) publishes original research in all aspects of Biochemistry, Biophysics and related areas like Molecular and Cell Biology. BB Reports welcomes solid though more preliminary, descriptive and small scale results if they have the potential to stimulate and/or contribute to future research, leading to new insights or hypothesis. Primary criteria for acceptance is that the work is original, scientifically and technically sound and provides valuable knowledge to life sciences research. We strongly believe all results deserve to be published and documented for the advancement of science. BB Reports specifically appreciates receiving reports on: Negative results, Replication studies, Reanalysis of previous datasets.