Combined transcriptome and physiological analysis reveals exogenous sucrose enhances photosynthesis and source capacity in foxtail millet

IF 6.1 2区 生物学 Q1 PLANT SCIENCES
Mengmeng Sun , Yongchao Li , Yunhao Chen , Dan-Ying Chen , Haiyu Wang , Jianhong Ren , Meijun Guo , Shuqi Dong , Xiaorui Li , Guanghui Yang , Lulu Gao , Xiaoqian Chu , Jia-Gang Wang , Xiangyang Yuan
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引用次数: 0

Abstract

Foxtail millet (Setaria italica (L.) P. Beauv.) is an environmentally friendly crop that meets the current requirements of international food security and is widely accepted as a photosynthesis research model. However, whether exogenous sucrose treatment has a positive effect on foxtail millet growth remains unknown. Here, we employed physiological and molecular approaches to identify photosynthesis and source capacity associated with exogenous sucrose during the growth of Jingu 21 seedlings. RNA-seq analysis showed that some differentially expressed genes (DEGs) related to photosynthesis and carotenoid biosynthesis were induced by exogenous sucrose and that most of these genes were up-regulated. An increase in gas exchange parameters, chlorophyll content, and chlorophyll fluorescence of Jingu 21 was noted after exogenous sucrose addition. Furthermore, exogenous sucrose up-regulated genes encoding sucrose and hexose transporters and enhanced starch and sucrose metabolism. More DEGs were up-regulated by sucrose, the nonstructural carbohydrate (NSC) content in the leaves increased and energy metabolism and sucrose loading subsequently improved, ultimately enhancing photosynthesis under normal and dark conditions. Further analysis revealed that WRKYs, ERFs, HY5, RAP2, and ABI5 could be key transcription factors involved in growth regulation. These results indicate that exogenous sucrose affects the normal photosynthetic performance of foxtail millet by increasing NSC transport and loading. They improve our understanding of the molecular mechanisms of the effects of exogenous sucrose on photosynthesis in foxtail millet, providing an effective measure to enhance source–sink relationships and improve yield.
转录组和生理分析相结合揭示外源蔗糖可提高狐尾黍的光合作用和源能力
狐尾黍(Setaria italica (L.) P. Beauv.)是一种符合当前国际粮食安全要求的环境友好型作物,被广泛接受为光合作用研究模型。然而,外源蔗糖处理是否会对狐尾粟的生长产生积极影响仍是未知数。在此,我们采用生理和分子方法鉴定了金谷21号幼苗生长过程中与外源蔗糖相关的光合作用和源能力。RNA-seq分析表明,外源蔗糖诱导了一些与光合作用和类胡萝卜素生物合成相关的差异表达基因(DEGs),而且这些基因中的大多数都被上调。添加外源蔗糖后,金谷 21 号的气体交换参数、叶绿素含量和叶绿素荧光均有所增加。此外,外源蔗糖还上调了编码蔗糖和己糖转运体的基因,促进了淀粉和蔗糖的新陈代谢。蔗糖上调了更多的 DEGs,叶片中的非结构碳水化合物(NSC)含量增加,能量代谢和蔗糖负荷随之改善,最终提高了正常和黑暗条件下的光合作用。进一步分析发现,WRKYs、ERFs、HY5、RAP2 和 ABI5 可能是参与生长调控的关键转录因子。这些结果表明,外源蔗糖通过增加 NSC 的运输和负载影响狐尾粟的正常光合作用。这些结果加深了我们对外源蔗糖影响狐尾粟光合作用的分子机制的理解,为改善源-汇关系和提高产量提供了有效措施。
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来源期刊
Plant Physiology and Biochemistry
Plant Physiology and Biochemistry 生物-植物科学
CiteScore
11.10
自引率
3.10%
发文量
410
审稿时长
33 days
期刊介绍: Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.
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