Cold atmospheric plasma as a promising tool in treatment of Trichophyton rubrum-induced skin infection in a guinea pig model of experimental dermatophytosis

IF 2.1 4区医学 Q3 INFECTIOUS DISEASES

Diagnostic microbiology and infectious disease Pub Date : 2024-10-09 DOI:10.1016/j.diagmicrobio.2024.116555

Asal Safi-Samghabadi , Seyed-Mohammad Atyabi , Masoomeh Shams-Ghahfarokhi , Zahra Salehi , Ali Eslamifar , Fatemehsadat Jamzivar , Mehdi Razzaghi-Abyaneh

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Abstract

The emergence of high-resistance strains to known antifungal drugs has highlighted the urgency of developing novel therapies for chronic dermatophytosis as a global health problem. An experimental dermatophytosis model in guinea pigs was developed to investigate the in vivo wound healing effects of cold atmospheric plasma (CAP) on T. rubrum skin invasion. Guinea pigs were experimentally infected with T. rubrum and wound healing was evaluated at 1, 4, 8 and 12 days post infection in the CAP-treated, terbinafine-treated and non-treated controls. Our results showed that CAP strongly inhibited the fungal virulence in vitro in culture media and in vivo on the skin lesions of experimentally infected guinea pigs even more efficient than that of terbinafine, resulting in complete wound healing at 8 days post infection. These results indicate that CAP would be considered as a promising tool comparable to conventional chemical therapies, for the treatment of drug-resistant chronic dermatophytosis caused by T. rubrum.

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冷大气等离子体是治疗豚鼠实验性皮肤癣菌病模型中由红色毛癣菌引起的皮肤感染的有效工具

对已知抗真菌药物产生高耐药性的菌株的出现，凸显了针对慢性皮癣这一全球性健康问题开发新型疗法的紧迫性。为了研究冷大气等离子体（CAP）对红念珠菌皮肤侵袭的体内伤口愈合效果，我们开发了一种豚鼠皮癣实验模型。实验中，豚鼠感染了红癣菌，并在感染后 1、4、8 和 12 天分别对 CAP 处理组、特比萘芬处理组和未处理组对照进行了伤口愈合评估。我们的结果表明，CAP 在体外培养基和体内实验感染豚鼠的皮损上都能有效抑制真菌的毒力，甚至比特比萘芬更有效，从而使伤口在感染后 8 天完全愈合。这些结果表明，在治疗由红念珠菌引起的耐药性慢性皮癣菌病方面，CAP 可被视为一种与传统化学疗法相媲美的有前途的工具。

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来源期刊

Diagnostic microbiology and infectious disease 医学-传染病学

CiteScore

5.30

自引率

3.40%

发文量

149

审稿时长

56 days

期刊介绍： Diagnostic Microbiology and Infectious Disease keeps you informed of the latest developments in clinical microbiology and the diagnosis and treatment of infectious diseases. Packed with rigorously peer-reviewed articles and studies in bacteriology, immunology, immunoserology, infectious diseases, mycology, parasitology, and virology, the journal examines new procedures, unusual cases, controversial issues, and important new literature. Diagnostic Microbiology and Infectious Disease distinguished independent editorial board, consisting of experts from many medical specialties, ensures you extensive and authoritative coverage.