The effect of curcumin 1% methanolic extract on the expression of Matrix Metalloproteinase-1, Matrix Metalloproteinase-8, Matrix Metalloproteinase-13, neutrophil, macrophage, lymphocyte counts in Porphyromonas gingivalis induced periodontitis: a randomized controlled trial

IF 1.7 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Saudi Dental Journal Pub Date : 2024-10-01 DOI:10.1016/j.sdentj.2024.08.002

Chika Rani Safitri , Wiet Sidharta , Artanti Kusumaningsih , Farisa Miyuki Wicaksono , M. Aljunaid , Agung Krismariono

{"title":"The effect of curcumin 1% methanolic extract on the expression of Matrix Metalloproteinase-1, Matrix Metalloproteinase-8, Matrix Metalloproteinase-13, neutrophil, macrophage, lymphocyte counts in Porphyromonas gingivalis induced periodontitis: a randomized controlled trial","authors":"Chika Rani Safitri , Wiet Sidharta , Artanti Kusumaningsih , Farisa Miyuki Wicaksono , M. Aljunaid , Agung Krismariono","doi":"10.1016/j.sdentj.2024.08.002","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><div>Hailed as one of the most fundamental and important treatment in management of periodontal disease, scalling and root planning has limitations regarding microorganism elimination. Meanwhile, turmeric has been proven to have a therapeutic effect on gingivitis and periodontitis. This study aimed to analyze the impact of curcumin 1% methanolic extract on the expression of Matrix Metalloproteinase-1, Matrix Metalloproteinase-8, Matrix Metalloproteinase -13, neutrophil, macrophage, and lymphocyte number in the gingiva of Wistar rats exposed to <em>Porphyromonas gingivalis bacteria</em> (<em>P. gingivalis</em>).</div></div><div><h3>Materials and methods</h3><div>The experimental animals used were Wistar rats, arbitrarilly split up into three experimental groups namely: normal, control (induced with periodontitis, received no treatment), and treatment (Periodontitis with 1% curcumin treatment). The samples were taken from the gingival tissue of the mandibular incisors on 1 and 7 days. Immunohistochemical and Hematoxylin Eosin staining were performed to measure the expression of Matrix Metalloproteinase-1, Matrix Metalloproteinase-8, Matrix Metalloproteinase-13, and the number of neutrophils, macrophages, and lymphocytes. Data were analyzed for mean standard deviation. The Oneway ANOVA was then performed to see whether there is a significant difference between experimental groups, the test then commenced with Tukey’s Honestly Significant Difference test with a significance level (α=0.05).</div></div><div><h3>Results</h3><div>MMP-8, MMP-13, neutrophil, and lymphocyte numbers in the treatment groups were significantly lower with P<0.05 than in the control groups in the 1 and 7 days. Meanwhile, in MMP-1 and macrophage numbers the difference was deemed not significant when control and treatment groups are compared.</div></div><div><h3>Conclusion</h3><div>The administration of 1% curcumin can significantly reduce the expression of MMP-8, MMP-13, neutrophil and lymphocyte cell numbers, but there is no reducing the number of macrophage cells and MMP-1 expression in gingiva of Wistar rats exposed to <em>P. gingivalis</em> bacteria.</div></div>","PeriodicalId":47246,"journal":{"name":"Saudi Dental Journal","volume":"36 10","pages":"Pages 1339-1343"},"PeriodicalIF":1.7000,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Saudi Dental Journal","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1013905224002153","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}

引用次数: 0

Abstract

Objective

Hailed as one of the most fundamental and important treatment in management of periodontal disease, scalling and root planning has limitations regarding microorganism elimination. Meanwhile, turmeric has been proven to have a therapeutic effect on gingivitis and periodontitis. This study aimed to analyze the impact of curcumin 1% methanolic extract on the expression of Matrix Metalloproteinase-1, Matrix Metalloproteinase-8, Matrix Metalloproteinase -13, neutrophil, macrophage, and lymphocyte number in the gingiva of Wistar rats exposed to Porphyromonas gingivalis bacteria (P. gingivalis).

Materials and methods

The experimental animals used were Wistar rats, arbitrarilly split up into three experimental groups namely: normal, control (induced with periodontitis, received no treatment), and treatment (Periodontitis with 1% curcumin treatment). The samples were taken from the gingival tissue of the mandibular incisors on 1 and 7 days. Immunohistochemical and Hematoxylin Eosin staining were performed to measure the expression of Matrix Metalloproteinase-1, Matrix Metalloproteinase-8, Matrix Metalloproteinase-13, and the number of neutrophils, macrophages, and lymphocytes. Data were analyzed for mean standard deviation. The Oneway ANOVA was then performed to see whether there is a significant difference between experimental groups, the test then commenced with Tukey’s Honestly Significant Difference test with a significance level (α=0.05).

Results

MMP-8, MMP-13, neutrophil, and lymphocyte numbers in the treatment groups were significantly lower with P<0.05 than in the control groups in the 1 and 7 days. Meanwhile, in MMP-1 and macrophage numbers the difference was deemed not significant when control and treatment groups are compared.

Conclusion

The administration of 1% curcumin can significantly reduce the expression of MMP-8, MMP-13, neutrophil and lymphocyte cell numbers, but there is no reducing the number of macrophage cells and MMP-1 expression in gingiva of Wistar rats exposed to P. gingivalis bacteria.

查看原文本刊更多论文

姜黄素 1%甲醇提取物对牙龈卟啉单胞菌诱导的牙周炎患者基质金属蛋白酶-1、基质金属蛋白酶-8、基质金属蛋白酶-13、中性粒细胞、巨噬细胞、淋巴细胞计数的影响：随机对照试验

目的作为治疗牙周病最基本、最重要的治疗方法之一，刮治和根管治疗在清除微生物方面存在局限性。同时，姜黄已被证实对牙龈炎和牙周炎有治疗作用。本研究旨在分析姜黄素 1%甲醇提取物对暴露于牙龈卟啉单胞菌（P. gingivalis）的 Wistar 大鼠牙龈中基质金属蛋白酶-1、基质金属蛋白酶-8、基质金属蛋白酶-13 的表达，以及中性粒细胞、巨噬细胞和淋巴细胞数量的影响。材料和方法实验动物为 Wistar 大鼠，任意分为三个实验组，即正常组、对照组（诱发牙周炎，未接受任何治疗）和治疗组（牙周炎，1%姜黄素治疗）。分别于 1 天和 7 天从下颌切牙的牙龈组织中取样。通过免疫组化和苏木精染色法检测基质金属蛋白酶-1、基质金属蛋白酶-8、基质金属蛋白酶-13的表达，以及中性粒细胞、巨噬细胞和淋巴细胞的数量。数据以平均标准偏差进行分析。结果治疗组的 MMP-8、MMP-13、中性粒细胞和淋巴细胞数量在 1 天和 7 天内显著低于对照组，P<0.05。结论 1%姜黄素能显著降低暴露于牙龈脓疱病菌的 Wistar 大鼠牙龈中的 MMP-8、MMP-13、中性粒细胞和淋巴细胞的表达，但巨噬细胞的数量和 MMP-1 的表达没有降低。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Saudi Dental Journal DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.60

自引率

0.00%

发文量

审稿时长

22 weeks

期刊介绍： Saudi Dental Journal is an English language, peer-reviewed scholarly publication in the area of dentistry. Saudi Dental Journal publishes original research and reviews on, but not limited to: • dental disease • clinical trials • dental equipment • new and experimental techniques • epidemiology and oral health • restorative dentistry • periodontology • endodontology • prosthodontics • paediatric dentistry • orthodontics and dental education Saudi Dental Journal is the official publication of the Saudi Dental Society and is published by King Saud University in collaboration with Elsevier and is edited by an international group of eminent researchers.