Exploring AMR and virulence in Klebsiella pneumoniae isolated from humans and pet animals: A complement of phenotype by WGS-derived profiles in a One Health study in Egypt

IF 4.1 2区医学 Q1 INFECTIOUS DISEASES

One Health Pub Date : 2024-09-27 DOI:10.1016/j.onehlt.2024.100904

Enas A. Soliman , Alaa Saad , Ashraf A. Abd El Tawab , Fatma I. Elhofy , Amira M. Rizk , Manar Elkhayat , Tamara Kozytska , Majdil Ilyas , Marwa Bassiouny , Hanka Brangsch , Mathias W. Pletz , Heinrich Neubauer , Lisa D. Sprague , Gamal Wareth

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引用次数: 0

Abstract

Klebsiella pneumoniae is a ubiquitous nosocomial pathogen associated with various types of infections in hospitalized patients and different animal species. In the current study, 49 Klebsiella strains isolated from humans, dogs, and cats were investigated using NGS technology. MALDI-TOF failed to identify newly discovered K. variicola and K. quasipneumoniae isolates correctly. MLST analysis revealed different sequence types among K. pneumoniae isolates, and the most frequent STs were ST29, ST219, and ST37. Three ST23 that are generally known as hypervirulent type were identified but they lacked major discriminatory determinants for hypervirulent K. pneumoniae (hvKp). K. pneumoniae isolates showed high diversity, and several isolates from humans and animals were assigned to the same ST and were almost identical. Isolates from humans exhibited more pronounced resistance patterns compared to the animal isolates. High levels of resistance were observed for piperacillin, trimethoprim/sulfamethoxazole, and cephalosporins, and resistance to carbapenem compounds was only found in isolates of human origin. Three strains of human origin were extensively drug-resistant (XDR). A diverse range of resistance genes primarily confer resistance to beta-lactams., phenicol/quinolone, aminoglycoside, macrolide, sulfonamides, and fosfomycin were identified in silico. However, there were inconsistencies between the phenotypic characterization of isolates and the set of resistance genes detected in silico in this set of Klebsiella isolates. Further research using a larger number of isolates from various sources is necessary to fully comprehend the relationship between the presence of antimicrobial resistance determinants and phenotypic data. It is also necessary to monitor the spread of K. pneumoniae from a One Health perspective in Egypt.

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探索分离自人类和宠物的肺炎克雷伯氏菌的 AMR 和毒力：埃及 "一个健康 "研究中的 WGS 衍生特征对表型的补充

肺炎克雷伯菌是一种无处不在的鼻腔病原体，与住院病人和不同动物物种的各种感染有关。本研究利用 NGS 技术对从人类、狗和猫身上分离出的 49 株克雷伯氏菌进行了研究。MALDI-TOF 未能正确识别新发现的变异克雷伯菌和类肺炎克雷伯菌分离株。MLST 分析显示，肺炎克氏菌分离株中存在不同的序列类型，最常见的 ST 为 ST29、ST219 和 ST37。发现了三种通常被称为高病毒型的 ST23，但它们缺乏对高病毒型肺炎克氏菌（hvKp）的主要鉴别决定因素。肺炎克氏菌分离物显示出高度的多样性，一些来自人类和动物的分离物被归入同一 ST，且几乎完全相同。与动物分离物相比，人类分离物表现出更明显的耐药性模式。哌拉西林、三甲双氨/磺胺甲噁唑和头孢菌素的耐药性水平较高，而对碳青霉烯类化合物的耐药性仅出现在人源分离物中。三株人源菌株具有广泛耐药性（XDR）。硅学研究发现了多种耐药基因，主要包括对β-内酰胺类、苯酚/喹诺酮类、氨基糖苷类、大环内酯类、磺胺类和磷霉素的耐药基因。然而，在这组克雷伯氏菌分离物中，分离物的表型特征与在硅学中检测到的耐药基因集之间存在不一致。有必要使用更多不同来源的分离物开展进一步研究，以全面了解抗菌药耐药性决定因素的存在与表型数据之间的关系。此外，还有必要从 "一体健康 "的角度监测肺炎克雷伯菌在埃及的传播情况。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

One Health Medicine-Infectious Diseases

CiteScore

8.10

自引率

4.00%

发文量

审稿时长

18 weeks

期刊介绍： One Health - a Gold Open Access journal. The mission of One Health is to provide a platform for rapid communication of high quality scientific knowledge on inter- and intra-species pathogen transmission, bringing together leading experts in virology, bacteriology, parasitology, mycology, vectors and vector-borne diseases, tropical health, veterinary sciences, pathology, immunology, food safety, mathematical modelling, epidemiology, public health research and emergency preparedness. As a Gold Open Access journal, a fee is payable on acceptance of the paper. Please see the Guide for Authors for more information. Submissions to the following categories are welcome: Virology, Bacteriology, Parasitology, Mycology, Vectors and vector-borne diseases, Co-infections and co-morbidities, Disease spatial surveillance, Modelling, Tropical Health, Discovery, Ecosystem Health, Public Health.