Assessment of genetic diversity, tissue tropism, and antigenic properties of Grimsö betacoronavirus in Swedish bank voles (Clethrionomys glareolus)

IF 4.1 2区医学 Q1 INFECTIOUS DISEASES

One Health Pub Date : 2024-10-08 DOI:10.1016/j.onehlt.2024.100911

Santiago Fernández Morente , Jinlin Li , Anishia Wasberg , Inês R. Faria , Elin Economou Lundeberg , Bo Settergren , Åke Lundkvist , Jiaxin Ling

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引用次数: 0

Abstract

Zoonotic coronaviruses can transmit over species barriers and infect humans. To understand the zoonotic potential of a betacoronavirus, Grimsö virus (GRIV), we investigated the geographic distribution and tissue tropism of GRIV in Swedish bank voles (Clethrionomys glareolus), and the antigenicity of the nucleocapsid (N) protein. We screened the lung tissues from animals collected in the southern Sweden by RT-PCR with primers targeting the spike gene. Seven out of 74 animals were found to be positive. They are genetically close to GRIV from Grimsö, central Sweden. Positive rodents were studied for the tissue distribution of GRIV and GRIV RNA was mainly found in the respiratory tract. After three attempts of virus isolation were failed, we successfully established a Vero E6 cell line that stably expressed GRIV N protein, which has no cross-reactivity with patient serum containing antibodies against SARS-CoV-2, or with MERS-CoV. However, a low level of cross-reactivity to common cold coronaviruses was found, likely HCoV-OC43 or HCoV-HKU1, probably due to shared linear epitopes. With the high prevalence and the suggested respiratory transmission route, GRIV may have a high potential for spillover and cross-species transmission, and future serological screening of GRIV infections in domestic animals or humans will be needed.

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评估瑞典银行田鼠（Clethrionomys glareolus）中 Grimsö betacoronavirus 的遗传多样性、组织滋养和抗原特性

人畜共患冠状病毒可跨越物种间的障碍传播并感染人类。为了了解一种betacoronavirus--Grimsö病毒（GRIV）的人畜共患可能性，我们研究了GRIV在瑞典银行田鼠（Clethrionomys glareolus）中的地理分布和组织滋养性，以及核壳蛋白（N）的抗原性。我们用针对尖峰基因的引物通过 RT-PCR 对在瑞典南部采集的动物肺部组织进行了筛查。结果发现，74 只动物中有 7 只呈阳性。它们在基因上与瑞典中部格里姆斯（Grimsö）的 GRIV 相似。对阳性啮齿动物进行了 GRIV 组织分布研究，发现 GRIV RNA 主要存在于呼吸道。在三次病毒分离尝试失败后，我们成功地建立了稳定表达 GRIV N 蛋白的 Vero E6 细胞系，该细胞系与含有 SARS-CoV-2 抗体的患者血清或 MERS-CoV 没有交叉反应。不过，发现与普通感冒冠状病毒（可能是 HCoV-OC43 或 HCoV-HKU1）有低水平的交叉反应，这可能是由于共享的线性表位所致。GRIV的流行率很高，而且有呼吸道传播途径，因此很有可能发生外溢和跨物种传播，今后需要对家畜或人类的GRIV感染进行血清学筛查。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

One Health Medicine-Infectious Diseases

CiteScore

8.10

自引率

4.00%

发文量

审稿时长

18 weeks

期刊介绍： One Health - a Gold Open Access journal. The mission of One Health is to provide a platform for rapid communication of high quality scientific knowledge on inter- and intra-species pathogen transmission, bringing together leading experts in virology, bacteriology, parasitology, mycology, vectors and vector-borne diseases, tropical health, veterinary sciences, pathology, immunology, food safety, mathematical modelling, epidemiology, public health research and emergency preparedness. As a Gold Open Access journal, a fee is payable on acceptance of the paper. Please see the Guide for Authors for more information. Submissions to the following categories are welcome: Virology, Bacteriology, Parasitology, Mycology, Vectors and vector-borne diseases, Co-infections and co-morbidities, Disease spatial surveillance, Modelling, Tropical Health, Discovery, Ecosystem Health, Public Health.