Circulation of tick-borne pathogens in wildlife of the Republic of Korea

IF 4.1 2区 医学 Q1 INFECTIOUS DISEASES
Hye-ryung Byun , Seong-Ryeong Ji , Jun-Gu Kang , Chang-Yong Choi , Ki-Jeong Na , Jong-Taek Kim , Joon-Seok Chae
{"title":"Circulation of tick-borne pathogens in wildlife of the Republic of Korea","authors":"Hye-ryung Byun ,&nbsp;Seong-Ryeong Ji ,&nbsp;Jun-Gu Kang ,&nbsp;Chang-Yong Choi ,&nbsp;Ki-Jeong Na ,&nbsp;Jong-Taek Kim ,&nbsp;Joon-Seok Chae","doi":"10.1016/j.onehlt.2024.100913","DOIUrl":null,"url":null,"abstract":"<div><div>Habitat loss of wildlife and increased human activities in their habitat provide more opportunities for human-wild animal contact. These artificial environments influence humans by facilitating the transmission of tick-borne pathogens. Therefore, we aimed to detect and understand circulating tick-borne pathogens in the natural environment by analyzing blood and spleen samples of wild animals admitted to wildlife rescue centers in the Republic of Korea. In total, 376 samples were collected from 355 rescued wild animals immediately after their arrival or death. After DNA deoxyribonucleic acid and RNA extractions, reverse transcription polymerase chain reaction (RT-PCR) and nested PCR were conducted to detect target tick-borne pathogens. This study detected six positive samples of severe fever with thrombocytopenia syndrome virus (SFTSV), 146 <em>Anaplasma phagocytophilum</em>, 55 <em>Anaplasma bovis</em>, 19 <em>Rickettsia</em> spp., 45 <em>Borrelia theileri</em>, and 4 <em>Bartonella schoenbuchensis</em>. Among the positive samples, SFTSV was detected in one spleen sample from a Korean water deer, from which SFTSV was successfully isolated. After full genome sequencing, the L, M, and S segments all belonged to genotype B-3 and indicated 99.84 % ∼ 99.94 % similarity with SFTSV isolated from human serum. In conclusion, wild animals are potential reservoirs of tick-borne pathogens. Therefore, surveillance systems to prevent transmission among ticks, animals, and humans must be developed using the One Health concept.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"19 ","pages":"Article 100913"},"PeriodicalIF":4.1000,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"One Health","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2352771424002398","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
引用次数: 0

Abstract

Habitat loss of wildlife and increased human activities in their habitat provide more opportunities for human-wild animal contact. These artificial environments influence humans by facilitating the transmission of tick-borne pathogens. Therefore, we aimed to detect and understand circulating tick-borne pathogens in the natural environment by analyzing blood and spleen samples of wild animals admitted to wildlife rescue centers in the Republic of Korea. In total, 376 samples were collected from 355 rescued wild animals immediately after their arrival or death. After DNA deoxyribonucleic acid and RNA extractions, reverse transcription polymerase chain reaction (RT-PCR) and nested PCR were conducted to detect target tick-borne pathogens. This study detected six positive samples of severe fever with thrombocytopenia syndrome virus (SFTSV), 146 Anaplasma phagocytophilum, 55 Anaplasma bovis, 19 Rickettsia spp., 45 Borrelia theileri, and 4 Bartonella schoenbuchensis. Among the positive samples, SFTSV was detected in one spleen sample from a Korean water deer, from which SFTSV was successfully isolated. After full genome sequencing, the L, M, and S segments all belonged to genotype B-3 and indicated 99.84 % ∼ 99.94 % similarity with SFTSV isolated from human serum. In conclusion, wild animals are potential reservoirs of tick-borne pathogens. Therefore, surveillance systems to prevent transmission among ticks, animals, and humans must be developed using the One Health concept.
大韩民国野生动物中的蜱媒病原体循环
野生动物栖息地的丧失和人类在其栖息地活动的增加为人类与野生动物的接触提供了更多机会。这些人为环境通过促进蜱媒病原体的传播来影响人类。因此,我们旨在通过分析大韩民国野生动物救助中心收治的野生动物的血液和脾脏样本,检测和了解自然环境中循环的蜱媒病原体。总共从 355 只获救野生动物身上采集了 376 份样本,这些样本都是在它们到达或死亡后立即采集的。在提取 DNA 脱氧核糖核酸和 RNA 后,进行了逆转录聚合酶链反应(RT-PCR)和巢式 PCR,以检测目标蜱传病原体。这项研究检测出了 6 份严重发热伴血小板减少综合征病毒(SFTSV)阳性样本、146 份噬细胞嗜酸性阿纳疟原虫样本、55 份牛阿纳疟原虫样本、19 份立克次体属样本、45 份包柔氏螺旋体样本和 4 份弓形虫样本。在阳性样本中,有一份来自韩国水鹿的脾脏样本检测到 SFTSV,并成功从中分离出 SFTSV。经过全基因组测序,其 L、M 和 S 片段均属于 B-3 基因型,与从人类血清中分离出的 SFTSV 相似度为 99.84 % ∼ 99.94 %。总之,野生动物是蜱传病原体的潜在储库。因此,必须利用 "同一健康 "理念建立监测系统,以防止蜱虫、动物和人类之间的传播。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
One Health
One Health Medicine-Infectious Diseases
CiteScore
8.10
自引率
4.00%
发文量
95
审稿时长
18 weeks
期刊介绍: One Health - a Gold Open Access journal. The mission of One Health is to provide a platform for rapid communication of high quality scientific knowledge on inter- and intra-species pathogen transmission, bringing together leading experts in virology, bacteriology, parasitology, mycology, vectors and vector-borne diseases, tropical health, veterinary sciences, pathology, immunology, food safety, mathematical modelling, epidemiology, public health research and emergency preparedness. As a Gold Open Access journal, a fee is payable on acceptance of the paper. Please see the Guide for Authors for more information. Submissions to the following categories are welcome: Virology, Bacteriology, Parasitology, Mycology, Vectors and vector-borne diseases, Co-infections and co-morbidities, Disease spatial surveillance, Modelling, Tropical Health, Discovery, Ecosystem Health, Public Health.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信