Alexis Lefevre, Cristian Brandi, Adele De Ninno, Filippo Ruggiero, Enrico Verona, Michael Gauthier, Paolo Bisegna, Aude Bolopion, Federica Caselli
{"title":"Real-time impedance-activated dielectrophoretic actuation for reconfigurable manipulation of single flowing particles","authors":"Alexis Lefevre, Cristian Brandi, Adele De Ninno, Filippo Ruggiero, Enrico Verona, Michael Gauthier, Paolo Bisegna, Aude Bolopion, Federica Caselli","doi":"10.1039/d4lc00622d","DOIUrl":null,"url":null,"abstract":"This work presents an innovative all-electrical platform for selective single-particle manipulation. The platform combines microfluidic impedance cytometry for label-free particle characterization and dielectrophoresis for contactless multi-way particle separation. The microfluidic chip has a straightforward coplanar electrode layout and no particle pre-focusing mechanism is required. An original online algorithm analyzes the impedance signals of each incoming particle and regulates in real-time the dielectrophoretic voltages according to a desired control logic. As proof-of-concept, three operation modes are demonstrated on a mixture of 8, 10, and 12 µm diameter beads: (i) particle position swapping across channel axis, irrespective of particle size, (ii) size-based particle separation, irrespective of particle position, and (iii) sorting of a selected sequence of particles. As a perspective, the versatility of impedance cytometry and dielectrophoresis and the possibility to configure alternative control logics hold promises for advanced particle and cell manipulation.","PeriodicalId":85,"journal":{"name":"Lab on a Chip","volume":null,"pages":null},"PeriodicalIF":6.1000,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Lab on a Chip","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1039/d4lc00622d","RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0
Abstract
This work presents an innovative all-electrical platform for selective single-particle manipulation. The platform combines microfluidic impedance cytometry for label-free particle characterization and dielectrophoresis for contactless multi-way particle separation. The microfluidic chip has a straightforward coplanar electrode layout and no particle pre-focusing mechanism is required. An original online algorithm analyzes the impedance signals of each incoming particle and regulates in real-time the dielectrophoretic voltages according to a desired control logic. As proof-of-concept, three operation modes are demonstrated on a mixture of 8, 10, and 12 µm diameter beads: (i) particle position swapping across channel axis, irrespective of particle size, (ii) size-based particle separation, irrespective of particle position, and (iii) sorting of a selected sequence of particles. As a perspective, the versatility of impedance cytometry and dielectrophoresis and the possibility to configure alternative control logics hold promises for advanced particle and cell manipulation.
期刊介绍:
Lab on a Chip is the premiere journal that publishes cutting-edge research in the field of miniaturization. By their very nature, microfluidic/nanofluidic/miniaturized systems are at the intersection of disciplines, spanning fundamental research to high-end application, which is reflected by the broad readership of the journal. Lab on a Chip publishes two types of papers on original research: full-length research papers and communications. Papers should demonstrate innovations, which can come from technical advancements or applications addressing pressing needs in globally important areas. The journal also publishes Comments, Reviews, and Perspectives.