Autocatalytic DNA circuitries

IF 40.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Qiong Wu, Wei Xu, Jinhua Shang, Jiajing Li, Xiaoqing Liu, Fuan Wang and Jinghong Li
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引用次数: 0

Abstract

Autocatalysis, a self-sustained replication process where at least one of the products functions as a catalyst, plays a pivotal role in life's evolution, from genome duplication to the emergence of autocatalytic subnetworks in cell division and metabolism. Leveraging their programmability, controllability, and rich functionalities, DNA molecules have become a cornerstone for engineering autocatalytic circuits, driving diverse technological applications. In this tutorial review, we offer a comprehensive survey of recent advances in engineering autocatalytic DNA circuits and their practical implementations. We delve into the fundamental principles underlying the construction of these circuits, highlighting their reliance on DNAzyme biocatalysis, enzymatic catalysis, and dynamic hybridization assembly. The discussed autocatalytic DNA circuitry techniques have revolutionized ultrasensitive sensing of biologically significant molecules, encompassing genomic DNAs, RNAs, viruses, and proteins. Furthermore, the amplicons produced by these circuits serve as building blocks for higher-order DNA nanostructures, facilitating biomimetic behaviors such as high-performance intracellular bioimaging and precise algorithmic assembly. We summarize these applications and extensively address the current challenges, potential solutions, and future trajectories of autocatalytic DNA circuits. This review promises novel insights into the advancement and practical utilization of autocatalytic DNA circuits across bioanalysis, biomedicine, and biomimetics.

Abstract Image

Abstract Image

自动催化 DNA 电路
自催化是一种自我维持的复制过程,其中至少有一种产物起着催化剂的作用。从基因组复制到细胞分裂和新陈代谢中自催化子网络的出现,自催化在生命进化过程中发挥着举足轻重的作用。DNA 分子利用其可编程性、可控性和丰富的功能,已成为工程自催化电路的基石,推动了各种技术应用。在这篇教程综述中,我们将全面介绍自催化 DNA 电路工程的最新进展及其实际应用。我们深入探讨了构建这些电路的基本原理,强调了它们对 DNA 酶生物催化、酶催化和动态杂交组装的依赖。所讨论的自催化 DNA 电路技术彻底改变了对重要生物分子(包括基因组 DNA、RNA、病毒和蛋白质)的超灵敏传感。此外,这些电路产生的扩增子可作为高阶 DNA 纳米结构的构件,促进生物仿生行为,如高性能细胞内生物成像和精确算法组装。我们总结了这些应用,并广泛探讨了自催化 DNA 电路当前面临的挑战、潜在的解决方案和未来的发展轨迹。这篇综述为自催化 DNA 电路在生物分析、生物医学和生物仿生学领域的发展和实际应用提供了新的见解。
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来源期刊
Chemical Society Reviews
Chemical Society Reviews 化学-化学综合
CiteScore
80.80
自引率
1.10%
发文量
345
审稿时长
6.0 months
期刊介绍: Chemical Society Reviews is published by: Royal Society of Chemistry. Focus: Review articles on topics of current interest in chemistry; Predecessors: Quarterly Reviews, Chemical Society (1947–1971); Current title: Since 1971; Impact factor: 60.615 (2021); Themed issues: Occasional themed issues on new and emerging areas of research in the chemical sciences
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