Exploring Bioactive Compounds in Prosopis juliflora Extracts Through Comparative LC–MS Analysis

IF 1.1 4区 农林科学 Q3 PLANT SCIENCES
Sanjeev Jakatimath, K. C. Kiran Kumar, R. K. Mesta, Raghavendra S., Raghavendra G., Archana T. S., Devendra Kumar
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引用次数: 0

Abstract

The current study was carried out to assess the alkaloid-enriched constituents of Prosopis juliflora (Fabaceae) against bacterial blight of pomegranate. Utilising plant extracts for disease management offers an eco-friendly approach. In vitro assessment of the minimum inhibitory concentration (MIC) for alkaloid-enriched fractions from the leaves and inflorescence of P. juliflora revealed a 14 mm zone of inhibition, with the lowest MIC value being 30 mg/mL. Untargeted metabolomics analysis of alkaloid-rich fractions (ARF) from four different tissues of P. juliflora, subjected to LC–MS for alkaloid identification, detected a total of 1489 peaks in positive mode and 240 peaks in negative mode. The highest peaks were detected in the leaves, where 47 metabolites and 9 alkaloids were identified. Ellipticine and ormosanine were found in relatively high concentrations across all four tissues, as indicated by their peak areas. The inflorescence had significantly high concentrations of sparteine, solanidine-I and puberanidine compared with other parts. Additionally, acetylsolanine was more abundant in the inflorescence, while solasodine-I was more prevalent in the leaves. A substantial amount of strictosidine was also found in the inflorescence.

通过 LC-MS 比对分析探索糙叶树提取物中的生物活性化合物
本研究旨在评估富含生物碱的糙叶木(豆科)成分对石榴细菌性枯萎病的防治效果。利用植物提取物进行病害防治是一种生态友好型方法。在体外评估富含生物碱的石榴叶和花序萃取物的最低抑菌浓度(MIC)时发现,抑菌区为 14 毫米,最低 MIC 值为 30 毫克/毫升。对鳞茎四种不同组织的生物碱富集馏分(ARF)进行了非靶向代谢组学分析,并采用 LC-MS 进行生物碱鉴定,在阳性模式下共检测到 1489 个峰,在阴性模式下检测到 240 个峰。叶片中的峰值最高,共鉴定出 47 种代谢物和 9 种生物碱。在所有四种组织中都发现了浓度相对较高的椭圆氨酸和奥莫萨宁,这可以从它们的峰面积看出。与其他部位相比,花序中的斯巴替因、茄碱-I 和苦参碱的浓度明显较高。此外,乙酰索兰宁在花序中含量更高,而索拉索定-I 在叶片中含量更高。在花序中还发现了大量的严格苷。
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来源期刊
Journal of Phytopathology
Journal of Phytopathology 生物-植物科学
CiteScore
2.90
自引率
0.00%
发文量
88
审稿时长
4-8 weeks
期刊介绍: Journal of Phytopathology publishes original and review articles on all scientific aspects of applied phytopathology in agricultural and horticultural crops. Preference is given to contributions improving our understanding of the biotic and abiotic determinants of plant diseases, including epidemics and damage potential, as a basis for innovative disease management, modelling and forecasting. This includes practical aspects and the development of methods for disease diagnosis as well as infection bioassays. Studies at the population, organism, physiological, biochemical and molecular genetic level are welcome. The journal scope comprises the pathology and epidemiology of plant diseases caused by microbial pathogens, viruses and nematodes. Accepted papers should advance our conceptual knowledge of plant diseases, rather than presenting descriptive or screening data unrelated to phytopathological mechanisms or functions. Results from unrepeated experimental conditions or data with no or inappropriate statistical processing will not be considered. Authors are encouraged to look at past issues to ensure adherence to the standards of the journal.
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