Hong Gao, Rongshen Wang, Yuxin Wang, Huiting Li, Shuying Lan, Mengyao Zhao, Xiaoxiao Liu, Wanzhong Li
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引用次数: 0
Abstract
In LPS-stimulated mouse inflammation model, Laoshan black tea polysaccharides (BTPSs) showed significant anti-inflammatory activity. Three water-soluble acidic polysaccharides (BTPS 3-1, BTPS 3-2, and BTPS 3-3) were purified. They contained glucose (Glc), galactose (Gal), fucose (Fuc), rhamnose (Rha), glucuronic acid (GlcA), and mannose (Man) at molar ratios of 9.0 : 4.9 : 4.9 : 1.7 : 1.5 : 1.0, 9.5 : 5.3 : 4.9 : 1.7 : 1.6 : 1.0, and 5.3 : 3.5 : 2.0 : 2.1 : 0.9 : 1.0 with average molecular weights of 28.38, 14.39, and 11.00 kDa, respectively. Three polysaccharides reduced the levels of NO, TNF-α, and IL-6 in LPS-induced RAW 264.7 cells, and BTPS 3-2 had the strongest anti-inflammatory effects. Based on analyses of the monosaccharide composition, methylation, and NMR, the main chain of BTPS 3-2 was composed of ⟶4) -β-D-Glcp- (1⟶, ⟶2) -α-L-Fucp- (1⟶, and ⟶6) -β-D-Galp- (1⟶, and Rhap, GlcpA, and Manp units were attached as side chains to the backbone. BTPS 3-2 also downregulated the expression levels of p-NF-κB p65, p-IκBα, TLR4, MyD88, COX-2, TNF-α, and IL-6. BTPS 3-2 ameliorated LPS-induced inflammation by regulating the TLR4/MyD88/NF-κB signaling pathway.
期刊介绍:
The Journal of Food Biochemistry publishes fully peer-reviewed original research and review papers on the effects of handling, storage, and processing on the biochemical aspects of food tissues, systems, and bioactive compounds in the diet.
Researchers in food science, food technology, biochemistry, and nutrition, particularly based in academia and industry, will find much of great use and interest in the journal. Coverage includes:
-Biochemistry of postharvest/postmortem and processing problems
-Enzyme chemistry and technology
-Membrane biology and chemistry
-Cell biology
-Biophysics
-Genetic expression
-Pharmacological properties of food ingredients with an emphasis on the content of bioactive ingredients in foods
Examples of topics covered in recently-published papers on two topics of current wide interest, nutraceuticals/functional foods and postharvest/postmortem, include the following:
-Bioactive compounds found in foods, such as chocolate and herbs, as they affect serum cholesterol, diabetes, hypertension, and heart disease
-The mechanism of the ripening process in fruit
-The biogenesis of flavor precursors in meat
-How biochemical changes in farm-raised fish are affecting processing and edible quality