Preparation and immunological study of Ichthyophthirius multifiliis β-tubulin DNA vaccine in grass carp (Ctenopharyngodon idella)

IF 3.2 2区农林科学 Q1 FISHERIES

Aquaculture Reports Pub Date : 2024-10-09 DOI:10.1016/j.aqrep.2024.102415

Jiazheng Ye , Fangling Liu , Siqi Ren , Zhuo Cai , Kejing Huang , Xinrui Wu , Yurui Lin , Wanqing Tong , Qianhui Li , Shanjian Zheng

{"title":"Preparation and immunological study of Ichthyophthirius multifiliis β-tubulin DNA vaccine in grass carp (Ctenopharyngodon idella)","authors":"Jiazheng Ye , Fangling Liu , Siqi Ren , Zhuo Cai , Kejing Huang , Xinrui Wu , Yurui Lin , Wanqing Tong , Qianhui Li , Shanjian Zheng","doi":"10.1016/j.aqrep.2024.102415","DOIUrl":null,"url":null,"abstract":"<div><div>Grass carp, a common aquatic species in China, suffer significant annual losses due to infection by the parasitic ciliate <em>Ichthyophthirius multifiliis</em> (Ich), thus necessitating effective prevention and control measures. In the present study, the Ich <em>β-tubulin</em> gene was cloned and inserted into the eukaryotic expression vector pVAX1 plasmid to construct the Ich β-tubulin DNA vaccine (pVAX1-Bt). After two doses of the pVAX1-Bt vaccine, the relative percentage survival (RPS) reached 70.0 %, whereas the RPS after 2 doses of pVAX1 reached 10.0 %. The β-tubulin antibody, specific antibody IgM, and immune enzymes (MDA, SOD, CAT, ACP, AKP, and cortisol) were tested over13 weeks. The findings revealed a significant enhancement in the vaccinated fish compared to their unvaccinated counterparts, indicating that the vaccine can activate the humoral immune pathway and non-specific immunity in fish, thereby promoting the production of specific antibodies and memory B cells. Collectively, these results indicate that the Ich β-tubulin DNA vaccine has potential in treatment of Ich infection (ichthyophthiriasis).</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102415"},"PeriodicalIF":3.2000,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Aquaculture Reports","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2352513424005039","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FISHERIES","Score":null,"Total":0}

引用次数: 0

Abstract

Grass carp, a common aquatic species in China, suffer significant annual losses due to infection by the parasitic ciliate Ichthyophthirius multifiliis (Ich), thus necessitating effective prevention and control measures. In the present study, the Ich β-tubulin gene was cloned and inserted into the eukaryotic expression vector pVAX1 plasmid to construct the Ich β-tubulin DNA vaccine (pVAX1-Bt). After two doses of the pVAX1-Bt vaccine, the relative percentage survival (RPS) reached 70.0 %, whereas the RPS after 2 doses of pVAX1 reached 10.0 %. The β-tubulin antibody, specific antibody IgM, and immune enzymes (MDA, SOD, CAT, ACP, AKP, and cortisol) were tested over13 weeks. The findings revealed a significant enhancement in the vaccinated fish compared to their unvaccinated counterparts, indicating that the vaccine can activate the humoral immune pathway and non-specific immunity in fish, thereby promoting the production of specific antibodies and memory B cells. Collectively, these results indicate that the Ich β-tubulin DNA vaccine has potential in treatment of Ich infection (ichthyophthiriasis).

查看原文本刊更多论文

草鱼多鳍鱼虫β-微管蛋白DNA疫苗的制备和免疫学研究

草鱼是中国常见的水生物种，每年因寄生纤毛虫多纤毛虫（Ich）的感染而遭受重大损失，因此必须采取有效的防控措施。本研究克隆了Ich β-微管蛋白基因，并将其插入真核表达载体pVAX1质粒中，构建了Ich β-微管蛋白DNA疫苗（pVAX1-Bt）。接种两剂pVAX1-Bt疫苗后，相对存活率（RPS）达到70.0%，而接种两剂pVAX1疫苗后的相对存活率为10.0%。在13周的时间里，对β-微管蛋白抗体、特异性抗体IgM和免疫酶（MDA、SOD、CAT、ACP、AKP和皮质醇）进行了检测。结果显示，与未接种疫苗的鱼类相比，接种疫苗的鱼类免疫力明显提高，这表明疫苗可以激活鱼类的体液免疫途径和非特异性免疫，从而促进特异性抗体和记忆性 B 细胞的产生。总之，这些结果表明，Ich β-tubulin DNA 疫苗具有治疗 Ich 感染（鱼鳞病）的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Aquaculture Reports Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

5.90

自引率

8.10%

发文量

469

审稿时长

77 days

期刊介绍： Aquaculture Reports will publish original research papers and reviews documenting outstanding science with a regional context and focus, answering the need for high quality information on novel species, systems and regions in emerging areas of aquaculture research and development, such as integrated multi-trophic aquaculture, urban aquaculture, ornamental, unfed aquaculture, offshore aquaculture and others. Papers having industry research as priority and encompassing product development research or current industry practice are encouraged.