Effects of dietary protein level on growth performance, plasma parameters, nutritional metabolism, and mTOR pathway of Chinese perch(Siniperca chuatsi)

IF 3.2 2区农林科学 Q1 FISHERIES

Aquaculture Reports Pub Date : 2024-10-02 DOI:10.1016/j.aqrep.2024.102390

Qiwei Zhang , Jiao Li , Liyun Ding , Yanping Zhang , Zhouming Qian , Xu-Fang Liang

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Abstract

This study aimed to investigate the impact of dietary protein levels (DPLs) on the growth performance, plasma parameters, nutritional metabolism, and the mTOR pathway in Chinese perch. Three replicates of Chinese perch (34.39 ± 0.16 g) were provided with five diets containing varying protein levels (39.99 %, 43.09 %, 46.04 %, 49.14 %, and 52.12 %) over an 8-week period. The results revealed that, as DPLs increased, the final weight (FW), daily weight gain (DWG)), specific growth rate (SGR) and protein efficiency ratio (PER) initially rose and then declined, reaching their peak at 49.14 % DPL group. The feed conversion ratio (FCR) decreased first and then increased. In addition, compared to 46.04 % DPL group, the 39.99 % DPL group significantly suppressed whole body crude protein, liver crude protein, and liver crude lipid contents. The 52.12 % DPL group significantly increased liver crude lipid content and aspartate aminotransferase (AST) activity, and increased plasma total cholesterol (TC), total triglyceride (TG) and total lipase (TL) concentrations. However, in Chinese perch fed with 46.04 % DPL, the expression of genes associated with liver amino acid catabolism (ast, gdh and ampd), lipid hydrolysis, and oxidation (hsl, lpl and cs), as well as glycolysis (pk), was significantly increased. And the expression levels of mTOR pathway-related genes (s6k and mtor) and the phosphorylation level of p-S6 were significantly elevated in the liver of fish fed with 46.04 % DPL. In summary, a low DPL (39.99 %) significantly impedes the growth performance of Chinese perch, resulting in an elevated feed conversion ratio. And a high DPL (52.12 %) can induce lipid deposition in the liver of Chinese perch, increase metabolic stress, and lead to liver damage. Additionally, piecewise regression analysis and second-degree polynomial analysis revealed that optimal protein level in Chinese perch feed is suggested to range between 46.10 % and 48.71 %. This study provides partial theoretical basis for the design of commercial practical feed formulas for Chinese perch.

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日粮蛋白质水平对鲈鱼生长性能、血浆参数、营养代谢和mTOR通路的影响

本研究旨在探讨日粮蛋白质水平（DPLs）对中国鲈鱼生长性能、血浆参数、营养代谢和mTOR通路的影响。给三个重复的中国鲈鱼（34.39 ± 0.16 g）提供5种不同蛋白质水平的日粮（39.99 %、43.09 %、46.04 %、49.14 %和52.12 %），为期8周。结果表明，随着DPL的增加，最终体重（FW）、日增重（DWG）、特定生长率（SGR）和蛋白质效率比（PER）先上升后下降，在49.14 % DPL组达到峰值。饲料转化率（FCR）先下降后上升。此外，与 46.04 % DPL 组相比，39.99 % DPL 组显著降低了全身粗蛋白、肝脏粗蛋白和肝脏粗脂肪含量。52.12 % DPL 组明显增加了肝脏粗脂含量和天冬氨酸氨基转移酶（AST）活性，并增加了血浆总胆固醇（TC）、总甘油三酯（TG）和总脂肪酶（TL）浓度。然而，在饲喂 46.04 % DPL 的中国鲈鱼中，与肝脏氨基酸分解（ast、ghd 和 ampd）、脂质水解和氧化（hsl、lpl 和 cs）以及糖酵解（pk）相关的基因表达量显著增加。在喂食 46.04 % DPL 的鱼类肝脏中，mTOR 通路相关基因（s6k 和 mtor）的表达水平和 p-S6 的磷酸化水平明显升高。总之，低 DPL（39.99 %）会明显影响鲈鱼的生长性能，导致饲料转化率升高。而过高的 DPL（52.12 %）会诱发鲈鱼肝脏脂质沉积，增加代谢应激，导致肝脏损伤。此外，片断回归分析和二级多项式分析表明，鲈鱼饲料中的最佳蛋白质水平建议在 46.10 % 至 48.71 % 之间。这项研究为设计商业实用的鲈鱼饲料配方提供了部分理论依据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Aquaculture Reports Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

5.90

自引率

8.10%

发文量

469

审稿时长

77 days

期刊介绍： Aquaculture Reports will publish original research papers and reviews documenting outstanding science with a regional context and focus, answering the need for high quality information on novel species, systems and regions in emerging areas of aquaculture research and development, such as integrated multi-trophic aquaculture, urban aquaculture, ornamental, unfed aquaculture, offshore aquaculture and others. Papers having industry research as priority and encompassing product development research or current industry practice are encouraged.