Influence of dietary Bacillus amyloliquefaciens AV5 on growth, immune responses, and resistance to Streptococcus agalactiae in Nile tilapia (Oreochromis niloticus)

IF 3.2 2区农林科学 Q1 FISHERIES

Aquaculture Reports Pub Date : 2024-10-01 DOI:10.1016/j.aqrep.2024.102391

Vicent Michael Shija , Kwaku Amoah , Yi Li , Zhong Yong , Zhengsi Chen , Jia Cai

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引用次数: 0

Abstract

This study assessed the effect of Bacillus amyloliquefaciens AV5 on the diet of Nile tilapia, focusing on growth, immune responses, and resistance to Streptococcus agalactiae infection. Fingerlings of Oreochromis niloticus were split into three groups and fed diets with different concentrations of B. amyloliquefaciens AV5 for 30 days as follows: no AV5, G1:106 CFU/g, G2:108 CFU/g. Upon completion of the feeding trial, G2 exhibited improved growth performance, including Wf, WGR, and SGR, along with a significantly lower (p< 0.05) feed conversion ratio (FCR) than the other groups. Enhanced immune and biochemical parameters (p < 0.05), including lysozyme (LZM), immunoglobulin M (IgM), alkaline phosphatase (AKP), and acid phosphatase (ACP), as well as antioxidant enzymes (superoxide dismutase (SOD) and catalase (CAT)) and digestive enzymes (trypsin (TYS), lipase (LPS), and protease (PTS)), were observed in group G2 compared to the GC group. No Significant differences in amylase (AML), cellulase (CLS), and xylanase (XYN) levels were observed among the GC, G1, and G2 groups. A substantial decrease (p< 0.05) in GOT, GPT, and MDA activity was observed in G2 compared to that in the GC group. No substantial differences in glucose (GL) levels were observed among the GC, G1, and G2 groups. A substantial increase (p< 0.05) in total protein (TP) was observed in G2 relative to the GC and G1 groups. Significant increases (p< 0.05) were observed in the liver and kidneys of growth- and immunity-related genes in the G2 group, including C-LYZ, GHR-1, TNF-α, IL-1β, and IGF-1. After intraperitoneal injection of S. agalactiae into both control and test fish, the cumulative mortalities in the GC, G1, and G2 groups were 78 %, 51 %, and 14 %, respectively. The findings indicate that administering B. amyloliquefaciens AV5 at a concentration of 1×10⁸cfu/g could potentially yield beneficial outcomes as a probiotic in aquaculture.

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膳食淀粉样芽孢杆菌 AV5 对尼罗罗非鱼（Oreochromis niloticus）生长、免疫反应和抗变形链球菌能力的影响

本研究评估了淀粉样芽孢杆菌 AV5 对尼罗罗非鱼日粮的影响，重点关注生长、免疫反应和对无乳链球菌感染的抵抗力。将尼罗罗非鱼幼苗分成三组，分别喂食含有不同浓度淀粉芽孢杆菌 AV5 的日粮 30 天：无 AV5、G1:106 CFU/g、G2:108 CFU/g。饲喂试验结束后，G2 的生长性能（包括 Wf、WGR 和 SGR）得到改善，饲料转化率（FCR）显著低于其他组（p< 0.05）。免疫和生化指标（p< 0.05），包括溶菌酶（LZM）、免疫球蛋白 M（IgM）、碱性磷酸酶（AKP）和酸性磷酸酶（ACP），以及抗氧化酶（超氧化物歧化酶（SOD）和过氧化氢酶（CAT））和消化酶（胰蛋白酶（TYS）、脂肪酶（LPS）和蛋白酶（PTS））。淀粉酶（AML）、纤维素酶（CLS）和木聚糖酶（XYN）水平在 GC 组、G1 组和 G2 组之间无明显差异。与 GC 组相比，G2 组的 GOT、GPT 和 MDA 活性大幅降低（p< 0.05）。GC 组、G1 组和 G2 组的葡萄糖（GL）水平没有明显差异。与 GC 组和 G1 组相比，在 G2 组观察到总蛋白 (TP) 显著增加（p< 0.05）。在 G2 组的肝脏和肾脏中观察到生长和免疫相关基因显著增加（p< 0.05），包括 C-LYZ、GHR-1、TNF-α、IL-1β 和 IGF-1。对照组和试验鱼腹腔注射 S. agalactiae 后，GC 组、G1 组和 G2 组的累积死亡率分别为 78%、51% 和 14%。研究结果表明，在水产养殖中使用浓度为 1×108cfu/g 的淀粉样芽孢杆菌 AV5 作为益生菌可能会产生有益的结果。

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来源期刊

Aquaculture Reports Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

5.90

自引率

8.10%

发文量

469

审稿时长

77 days

期刊介绍： Aquaculture Reports will publish original research papers and reviews documenting outstanding science with a regional context and focus, answering the need for high quality information on novel species, systems and regions in emerging areas of aquaculture research and development, such as integrated multi-trophic aquaculture, urban aquaculture, ornamental, unfed aquaculture, offshore aquaculture and others. Papers having industry research as priority and encompassing product development research or current industry practice are encouraged.