In situ phosphorus-modified Mg2Ge/Zn-Cu composite with improved mechanical, degradation, biotribological properties, and in vitro and in vivo osteogenesis and osteointegration performance for biodegradable bone-implant applications

IF 18 1区医学 Q1 ENGINEERING, BIOMEDICAL

Bioactive Materials Pub Date : 2024-10-11 DOI:10.1016/j.bioactmat.2024.09.026

Xian Tong , Xinkun Shen , Zhiqiang Lin , Runqi Zhou , Yue Han , Li Zhu , Shengbin Huang , Jianfeng Ma , Yuncang Li , Cuie Wen , Jixing Lin

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Abstract

Zinc (Zn)-based composites are promising biodegradable bone-implant materials because of their good biocompatibility, processability, and biodegradability. Nevertheless, the low interfacial bonding strength, coordinated deformation capacity, and mechanical strength of current Zn-based composites hinder their clinical application. In this study, we developed a biodegradable in situ 4Mg₂Ge/Zn-0.3Cu-0.05P composite (denoted ZMGCP) via phosphorus (P) modification and hot-rolling for bone-implant applications. The mechanical properties, corrosion behavior, biotribological performance, in vitro cytocompatibility and osteogenic differentiation, and in vivo osteogenesis and osteointegration of the as-cast (AC) and hot-rolled (HR) ZMGCP samples were systematically evaluated and compared to those of 4Mg₂Ge/Zn-0.3Cu (denoted ZMGC). The primary and eutectic reinforcement Mg₂Ge phases formed during solidification were refined after P modification and hot-rolling. The HR ZMGCP exhibited the best tensile properties among all the samples with an ultimate tensile strength of 288.9 MPa, a yield strength of 194.5 MPa, and an elongation of 17.7 %. The HR ZMGCP showed the lowest corrosion rate of 336 μm/a, 186 μm/a, and 61.7 μm/a as measured by potentiodynamic polarization, electrochemical impedance spectroscopy, and immersion testing, respectively, among all the samples in Hanks’ solution. The HR ZMGCP also showed higher biotribological resistance than its ZMGC counterpart. The HR ZMGCP exhibited the highest in vitro cytocompatibility, the best osteogenesis capability and angiogenesis property among the HR samples of pure Zn, ZMGC, and ZMGCP. Furthermore, the HR ZMGCP displayed complete in vivo biocompatibility, osteogenesis, osteointegration capability, and an appropriate degradation rate, showing significant potential for a biodegradable bone-implant material.

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原位磷改性 Mg2Ge/Zn-Cu 复合材料具有更好的机械、降解和生物ribological 性能，以及体内外骨生成和骨整合性能，可用于生物降解骨植入应用

锌（Zn）基复合材料具有良好的生物相容性、可加工性和生物降解性，是一种很有前景的可生物降解骨植入材料。然而，目前锌基复合材料的界面结合强度、协调变形能力和机械强度较低，阻碍了其临床应用。在本研究中，我们通过磷（P）改性和热轧技术开发了一种可生物降解的原位 4Mg2Ge/Zn-0.3Cu-0.05P 复合材料（简称 ZMGCP），用于骨植入应用。系统地评估了铸造（AC）和热轧（HR）ZMGCP 样品的力学性能、腐蚀行为、生物ribological 性能、体外细胞相容性和成骨分化，以及体内成骨和骨整合，并与 4Mg2Ge/Zn-0.3Cu （ZMGC）进行了比较。经过 P 改性和热轧后，凝固过程中形成的初级和共晶强化 Mg2Ge 相得到了细化。在所有样品中，HR ZMGCP 的拉伸性能最好，极限拉伸强度为 288.9 兆帕，屈服强度为 194.5 兆帕，伸长率为 17.7%。在汉克斯溶液中，通过电位极化、电化学阻抗光谱和浸泡试验测量，HR ZMGCP 的腐蚀速率在所有样品中最低，分别为 336 μm/a、186 μm/a 和 61.7 μm/a。与 ZMGC 相比，HR ZMGCP 还表现出更高的生物抗性。在纯 Zn、ZMGC 和 ZMGCP 的 HR 样品中，HR ZMGCP 表现出最高的体外细胞相容性、最佳的成骨能力和血管生成特性。此外，HR ZMGCP 在体内表现出完全的生物相容性、成骨能力、骨整合能力和适当的降解率，显示出其作为可生物降解骨植入材料的巨大潜力。

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来源期刊

Bioactive Materials Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

28.00

自引率

6.30%

发文量

436

审稿时长

20 days

期刊介绍： Bioactive Materials is a peer-reviewed research publication that focuses on advancements in bioactive materials. The journal accepts research papers, reviews, and rapid communications in the field of next-generation biomaterials that interact with cells, tissues, and organs in various living organisms. The primary goal of Bioactive Materials is to promote the science and engineering of biomaterials that exhibit adaptiveness to the biological environment. These materials are specifically designed to stimulate or direct appropriate cell and tissue responses or regulate interactions with microorganisms. The journal covers a wide range of bioactive materials, including those that are engineered or designed in terms of their physical form (e.g. particulate, fiber), topology (e.g. porosity, surface roughness), or dimensions (ranging from macro to nano-scales). Contributions are sought from the following categories of bioactive materials: Bioactive metals and alloys Bioactive inorganics: ceramics, glasses, and carbon-based materials Bioactive polymers and gels Bioactive materials derived from natural sources Bioactive composites These materials find applications in human and veterinary medicine, such as implants, tissue engineering scaffolds, cell/drug/gene carriers, as well as imaging and sensing devices.