Homogenization-circulating ultrasound in combination with aqueous enzymatic pretreatment for microwave-assisted extraction of kernel oil and essential oil from the fruit of Litsea cubeba

IF 8.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry Pub Date : 2024-10-10 DOI:10.1016/j.ultsonch.2024.107093

Xinyu Yang , Yang Yang , Ke Zhang , Ru Zhao , Hao Tian , Lei Yang , Xiuhua Zhao

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引用次数: 0

Abstract

Homogenization-circulating ultrasound in combination with an aqueous enzymatic pretreatment microwave-assisted extraction (HCUEPM) technique was successfully applied to extract kernel oil and essential oil from Litsea cubeba fruits. After screening the types and concentrations of enzymes, a 3 % pectinase aqueous solution was chosen. The Plackett–Burman design was used to screen eight parameters that might affect the yield of kernel oil and essential oil to identify significant variables. The best conditions were then predicted by further optimizing statistically significant factors via the Box–Behnken design. The optimal conditions were as follows: stirring speed of 1000 r/min, environmental pH of 5, homogenization time of 4 min, duty cycle of 20 %, ultrasound irradiation power of 400 W, incubation temperature of 52.78 °C, liquid–solid ratio of 9.31 mL/g, and incubation time of 2.53 h. Three parallel experiments were conducted under these conditions, yielding actual kernel oil at 240.56 ± 11.07 mL/kg DW and essential oil at 64.89 ± 3.1 mL/kg DW, which are close to the theoretical values. Compared with the HCUEPM method, the homogenization-microwave-assisted hydrodistillation (HMHD) method yielded 65.63 ± 3.2 mL/kg DW of essential oil but could not extract kernel oil. These findings demonstrate that the HCUEPM used in this study can efficiently extract a significant amount of kernel oil and essential oil from L. cubeba fruits in a short period of time. GC–MS analysis of the kernel oil and essential oil extracted via different methods revealed no significant differences in composition. The main components of the essential oil were D-limonene, trans-citral, cis-citral, and citronellal. The main components of the kernel oil were C₁₀ and C₁₂ medium-chain fatty acids, laying the foundation for the potential application of L. cubeba kernel oil and essential oil in the field of human health.

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匀化-循环超声与水酶法预处理相结合，用于微波辅助提取岩白菜果实中的核仁油和精油

匀浆-循环超声波结合水酶法预处理微波辅助萃取（HCUEPM）技术被成功地应用于从立木香果中萃取核仁油和精油。在对酶的种类和浓度进行筛选后，选择了 3% 的果胶酶水溶液。采用普拉克特-伯曼设计筛选了可能影响果仁油和精油产量的八个参数，以确定重要的变量。然后，通过箱-贝肯设计进一步优化统计意义上的重要因素，预测出最佳条件。最佳条件如下：搅拌速度为 1000 r/min，环境 pH 值为 5，均质时间为 4 min，占空比为 20%，超声波照射功率为 400 W，培养温度为 52.78 °C，液固比为 9.31 mL/g，培养时间为 2.53 h。在这些条件下进行了三次平行实验，实际核仁油的产量为 240.56 ± 11.07 mL/kg DW，精油的产量为 64.89 ± 3.1 mL/kg DW，均接近理论值。与 HCUEPM 方法相比，均质-微波辅助水蒸馏（HMHD）方法的精油产量为 65.63 ± 3.2 mL/kg DW，但不能提取核仁油。这些结果表明，本研究中使用的 HCUEPM 能在短时间内从立方体果实中有效提取大量的果仁油和精油。通过不同方法提取的果仁油和精油的气相色谱-质谱（GC-MS）分析表明，其成分没有明显差异。精油的主要成分是 D-柠檬烯、反式柠檬醛、顺式柠檬醛和香茅醛。核仁油的主要成分是 C10 和 C12 中链脂肪酸，这为立方体核仁油和精油在人类健康领域的潜在应用奠定了基础。

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来源期刊

Ultrasonics Sonochemistry 化学-化学综合

CiteScore

15.80

自引率

11.90%

发文量

361

审稿时长

59 days

期刊介绍： Ultrasonics Sonochemistry stands as a premier international journal dedicated to the publication of high-quality research articles primarily focusing on chemical reactions and reactors induced by ultrasonic waves, known as sonochemistry. Beyond chemical reactions, the journal also welcomes contributions related to cavitation-induced events and processing, including sonoluminescence, and the transformation of materials on chemical, physical, and biological levels. Since its inception in 1994, Ultrasonics Sonochemistry has consistently maintained a top ranking in the "Acoustics" category, reflecting its esteemed reputation in the field. The journal publishes exceptional papers covering various areas of ultrasonics and sonochemistry. Its contributions are highly regarded by both academia and industry stakeholders, demonstrating its relevance and impact in advancing research and innovation.