Biodegradable soy protein isolate carvacrol coating controls soft rot on nectarines by enhancing defense response

IF 2.7 2区农林科学 Q1 ENTOMOLOGY

Journal of Stored Products Research Pub Date : 2024-10-08 DOI:10.1016/j.jspr.2024.102444

{"title":"Biodegradable soy protein isolate carvacrol coating controls soft rot on nectarines by enhancing defense response","authors":"","doi":"10.1016/j.jspr.2024.102444","DOIUrl":null,"url":null,"abstract":"<div><div>Nectarine fruits are rich in nutrients, which are susceptible to fungal infection during postharvest, resulting in quality deterioration and decay. This study aimed to assess the effect of different concentrations of carvacrol and soy protein isolate coatings on the fungal infection and quality characteristics of nectarines during postharvest storage. The results showed that soy protein isolate carvacrol (SPI-C) coating inhibited the growth of <em>Rhizopus stolonifer</em> in vitro. The SPI-C coating significantly reduced the incidence of postharvest soft rot, maintained the postharvest quality of nectarines during storage. SPI-C coating induces the defense response to soft rot by enhancing the accumulation of phenolic, flavonoids and lignin, and up-regulating the expression of defense-related genes in nectarines. The SPI-C coating activated the transcription of antioxidant enzymes (CAT, SOD and POD) and eliminated ROS damage. The disease resistance response promotes the production of defense-related substances by upregulating the gene expression of <em>PpPPO</em> and <em>PpPAL</em>. In summary, SPI-C coating significantly reduces the incidence of postharvest soft rot, maintains the postharvest quality and induces defense response to soft rot by enhancing the expression of genes involved in phenylpropanoid metabolism in nectarines during storage.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":null,"pages":null},"PeriodicalIF":2.7000,"publicationDate":"2024-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Stored Products Research","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0022474X24002017","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENTOMOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Nectarine fruits are rich in nutrients, which are susceptible to fungal infection during postharvest, resulting in quality deterioration and decay. This study aimed to assess the effect of different concentrations of carvacrol and soy protein isolate coatings on the fungal infection and quality characteristics of nectarines during postharvest storage. The results showed that soy protein isolate carvacrol (SPI-C) coating inhibited the growth of Rhizopus stolonifer in vitro. The SPI-C coating significantly reduced the incidence of postharvest soft rot, maintained the postharvest quality of nectarines during storage. SPI-C coating induces the defense response to soft rot by enhancing the accumulation of phenolic, flavonoids and lignin, and up-regulating the expression of defense-related genes in nectarines. The SPI-C coating activated the transcription of antioxidant enzymes (CAT, SOD and POD) and eliminated ROS damage. The disease resistance response promotes the production of defense-related substances by upregulating the gene expression of PpPPO and PpPAL. In summary, SPI-C coating significantly reduces the incidence of postharvest soft rot, maintains the postharvest quality and induces defense response to soft rot by enhancing the expression of genes involved in phenylpropanoid metabolism in nectarines during storage.

查看原文本刊更多论文

可生物降解的大豆分离蛋白香芹酚涂层通过增强防御反应控制油桃软腐病的发生

油桃果实营养丰富，采后贮藏期间易受真菌感染，导致品质下降和腐烂。本研究旨在评估不同浓度的香芹酚和大豆分离蛋白包衣对油桃采后贮藏期间真菌感染和品质特征的影响。结果表明，大豆分离蛋白香芹酚（SPI-C）涂层能抑制体外根瘤菌的生长。SPI-C 涂层能明显降低采后软腐病的发生率，保持油桃在采后贮藏期间的品质。SPI-C涂层可通过提高油桃中酚类、黄酮类和木质素的积累以及上调防御相关基因的表达，诱导油桃对软腐病的防御反应。SPI-C 涂层激活了抗氧化酶（CAT、SOD 和 POD）的转录，消除了 ROS 损伤。抗病反应通过上调 PpPPO 和 PpPAL 的基因表达，促进防御相关物质的产生。总之，SPI-C 涂层能显著降低采后软腐病的发病率，保持采后品质，并通过提高贮藏期间油桃中参与苯丙氨酸代谢的基因的表达，诱导对软腐病的防御反应。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Stored Products Research 生物-昆虫学

CiteScore

5.70

自引率

18.50%

发文量

112

审稿时长

45 days

期刊介绍： The Journal of Stored Products Research provides an international medium for the publication of both reviews and original results from laboratory and field studies on the preservation and safety of stored products, notably food stocks, covering storage-related problems from the producer through the supply chain to the consumer. Stored products are characterised by having relatively low moisture content and include raw and semi-processed foods, animal feedstuffs, and a range of other durable items, including materials such as clothing or museum artefacts.