Volatiles potentially useful to be used as biomarkers for monitoring the khapra beetle Trogoderma granarium for quarantine

IF 2.7 2区农林科学 Q1 ENTOMOLOGY

Journal of Stored Products Research Pub Date : 2024-10-04 DOI:10.1016/j.jspr.2024.102430

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引用次数: 0

Abstract

The khapra beetle, Trogoderma granarium, is one of the most important quarantine pests globally. In order to identify potential biomarkers for establishing a rapid and effective monitoring technology for T. granarium quarantine, headspace-solid phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrum (GC-MS) was used to identify volatiles that are potentially useful for early detection of the presence of the T. granarium presence. Three volatiles, including Ethyl palmitate, (Z)-5-tetradecenoic acid and (Z)-14-methyl-8-hexadecen-1-al, were commonly identified as unique volatiles emitted from four different stored products including peanut, barley, wheat and rapeseed only after T. granarium infestation. In addition, four volatiles including hexadecane, palmitic acid, (Z)-14-methyl-8-hexadecen-1-al and N-heptadecane, were found emitted commonly from the larvae of T. granarium after they fed on the four stored products. These common chemicals could potentially be biomarkers sensitive enough for monitoring the presence of T. granarium. In addition to these unique chemicals, a combination of up- and down-regulated volatiles by each type of stored seeds were also identified as potential markers useful for the early detection of the presence of T. granarium. Our results could be a first step towards establishing a method for T. granarium monitoring and quarantine.

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可用作生物标记的挥发性物质，用于监测卡帕甲虫（Trogoderma granarium），以进行检疫

卡布拉甲虫（Trogoderma granarium）是全球最重要的检疫害虫之一。为了确定潜在的生物标志物，以建立快速有效的 T. granarium 检疫监测技术，研究人员利用顶空固相微萃取（HS-SPME）与气相色谱-质谱（GC-MS）联用技术，确定了可能有助于早期检测 T. granarium 是否存在的挥发性物质。三种挥发物，包括棕榈酸乙酯、(Z)-5-十四烯酸和(Z)-14-甲基-8-十六烯-1-醛，被普遍鉴定为仅在麦角菌侵染后才从四种不同的贮藏产品（包括花生、大麦、小麦和油菜籽）中释放的独特挥发物。此外，还发现谷斑皮蠹幼虫取食四种贮藏产品后通常会释放出四种挥发性物质，包括十六烷、棕榈酸、（Z）-14-甲基-8-十六烯-1-al 和 N-十七烷。这些常见的化学物质有可能成为生物标志物，对监测格氏梭菌的存在具有足够的灵敏度。除了这些独特的化学物质外，每种贮藏种子的上调和下调挥发物组合也被确定为可用于早期检测谷斑皮蠹存在的潜在标记物。我们的研究结果可能是建立 T. granarium 监测和检疫方法的第一步。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Stored Products Research 生物-昆虫学

CiteScore

5.70

自引率

18.50%

发文量

112

审稿时长

45 days

期刊介绍： The Journal of Stored Products Research provides an international medium for the publication of both reviews and original results from laboratory and field studies on the preservation and safety of stored products, notably food stocks, covering storage-related problems from the producer through the supply chain to the consumer. Stored products are characterised by having relatively low moisture content and include raw and semi-processed foods, animal feedstuffs, and a range of other durable items, including materials such as clothing or museum artefacts.