Metagenomic characterization of viruses in the serum of children with newly diagnosed cancer

IF 4 3区医学 Q2 VIROLOGY

Journal of Clinical Virology Pub Date : 2024-10-06 DOI:10.1016/j.jcv.2024.105736

Gustaf Leijonhufvud , Tatiany Aparecida Teixeira Soratto , Gabriel Machado Matos , Amanj Bajalan , Claudia Eichler-Jonsson , Britt Gustafsson , Gordana Bogdanovic , Tobias Allander , Gustaf Ljungman , Björn Andersson

{"title":"Metagenomic characterization of viruses in the serum of children with newly diagnosed cancer","authors":"Gustaf Leijonhufvud , Tatiany Aparecida Teixeira Soratto , Gabriel Machado Matos , Amanj Bajalan , Claudia Eichler-Jonsson , Britt Gustafsson , Gordana Bogdanovic , Tobias Allander , Gustaf Ljungman , Björn Andersson","doi":"10.1016/j.jcv.2024.105736","DOIUrl":null,"url":null,"abstract":"<div><h3>Background and Objectives</h3><div>A large cohort of pediatric patients with various forms of childhood cancer was investigated for the presence of viruses using metagenomics. A total of 476 patient samples, collected between 1989 and 2018, were analyzed, representing various pediatric oncological diagnoses and a control group of non-malignant diagnoses.</div></div><div><h3>Study design</h3><div>The study was carried out using metagenomic sequencing of serum samples. Viruses were identified and analyzed using bioinformatics methods, followed by Polymerase chain reaction (PCR) confirmation</div></div><div><h3>Results</h3><div>The results indicate that a wide range of viruses can be detected in the bloodstream of children with newly diagnosed cancer. Nine viral genomes were identified: Human Pegivirus (HPgV), Hepatitis C virus, Parechovirus 1, Rhinovirus C, Human papillomavirus 116, Human polyomavirus 10, Parvovirus B19, and different variants of Torque Teno Virus (TTV). In this study, a previously unknown virus was found belonging to the Iflavirdae family in the order Picornavirales. HPGV was significantly more common in patients with leukemia compared to other conditions.</div></div><div><h3>Conclusions</h3><div>These results highlight the abundance of systemic virus infections in children, and the value of metagenomic sequencing for hypothesis forming regarding the associations between virus infections and cancer.</div></div>","PeriodicalId":15517,"journal":{"name":"Journal of Clinical Virology","volume":"175 ","pages":"Article 105736"},"PeriodicalIF":4.0000,"publicationDate":"2024-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Clinical Virology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1386653224000982","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"VIROLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background and Objectives

A large cohort of pediatric patients with various forms of childhood cancer was investigated for the presence of viruses using metagenomics. A total of 476 patient samples, collected between 1989 and 2018, were analyzed, representing various pediatric oncological diagnoses and a control group of non-malignant diagnoses.

Study design

The study was carried out using metagenomic sequencing of serum samples. Viruses were identified and analyzed using bioinformatics methods, followed by Polymerase chain reaction (PCR) confirmation

Results

The results indicate that a wide range of viruses can be detected in the bloodstream of children with newly diagnosed cancer. Nine viral genomes were identified: Human Pegivirus (HPgV), Hepatitis C virus, Parechovirus 1, Rhinovirus C, Human papillomavirus 116, Human polyomavirus 10, Parvovirus B19, and different variants of Torque Teno Virus (TTV). In this study, a previously unknown virus was found belonging to the Iflavirdae family in the order Picornavirales. HPGV was significantly more common in patients with leukemia compared to other conditions.

Conclusions

These results highlight the abundance of systemic virus infections in children, and the value of metagenomic sequencing for hypothesis forming regarding the associations between virus infections and cancer.

查看原文本刊更多论文

新诊断癌症儿童血清中病毒的元基因组特征

背景与目的研究人员利用元基因组学方法对患有各种形式儿童癌症的大量儿科患者进行了调查，以确定是否存在病毒。共分析了 1989 年至 2018 年间收集的 476 份患者样本，这些样本代表了各种儿科肿瘤诊断和非恶性诊断对照组。结果结果表明，在新诊断癌症患儿的血液中可以检测到多种病毒。共鉴定出九种病毒基因组：人类佩吉病毒 (HPgV)、丙型肝炎病毒、帕雷奇病毒 1、鼻病毒 C、人类乳头瘤病毒 116、人类多瘤病毒 10、副病毒 B19 和托克特诺病毒 (TTV) 的不同变种。在这项研究中，发现了一种以前未知的病毒，属于短小病毒目伊夫拉病毒科。结论：这些结果凸显了儿童全身性病毒感染的广泛性，以及元基因组测序在病毒感染与癌症相关性假设形成方面的价值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Clinical Virology 医学-病毒学

CiteScore

22.70

自引率

1.10%

发文量

149

审稿时长

24 days

期刊介绍： The Journal of Clinical Virology, an esteemed international publication, serves as the official journal for both the Pan American Society for Clinical Virology and The European Society for Clinical Virology. Dedicated to advancing the understanding of human virology in clinical settings, the Journal of Clinical Virology focuses on disseminating research papers and reviews pertaining to the clinical aspects of virology. Its scope encompasses articles discussing diagnostic methodologies and virus-induced clinical conditions, with an emphasis on practicality and relevance to clinical practice. The journal publishes on topics that include: • new diagnostic technologies • nucleic acid amplification and serologic testing • targeted and metagenomic next-generation sequencing • emerging pandemic viral threats • respiratory viruses • transplant viruses • chronic viral infections • cancer-associated viruses • gastrointestinal viruses • central nervous system viruses • one health (excludes animal health)