Genome-wide identification and expression analysis of genes encoding late embryogenesis proteins in Cicer arietinum

IF 2.2 Q3 GENETICS & HEREDITY

Plant Gene Pub Date : 2024-09-28 DOI:10.1016/j.plgene.2024.100469

Reetu Singh , Varnika Rana , Sudesh Kumar Yadav , Vinay Kumar

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引用次数: 0

Abstract

Late embryogenesis abundant (LEA) proteins play defensive roles during seed maturation and seed germination processes. However, there is no such investigation was carried out in chickpea. In present study, genome wide identification and characterization of LEA encoding genes has been investigated, and identified 65 and 74 LEA encoding genes in desi and kabuli cultivar of chickpea, respectively. All these genes have been classified into eight subfamilies on the bases of their phylogenetic analysis and conserved domain. Maximum members of LEA encoding genes were found to be a part of the LEA_2 gene family. The analysis of physicochemical properties of LEAs was also conducted. LEA encoding genes have been found to be located in all chromosomes (8 chr) of chickpea and identified as involved in response to stimulus, biological processes, molecular functions and cellular components based upon gene ontology analysis. Gene expression analysis of randomly selected 8 LEA encoding genes has been carried out during different seed developmental stages which revealed the higher expression of LEA encoding genes during later stage of seed development in chickpea and proved their potential role in desiccation process during seed maturation. During seed germination, expression analysis of LEA encoding genes was found to be higher during the initial stages of seed germination. In conclusion, this work highlights the genome wide identification and characterization of LEA encoding genes in chickpea and proposed potential roles during seed developmental processes. This information could also be useful as a reference investigation for molecular breeding of chickpea for recalcitrant behaviour of seed.

查看原文本刊更多论文

对 Cicer arietinum 中编码胚胎后期发生蛋白的基因进行全基因组鉴定和表达分析

胚胎发生后期丰富蛋白（LEA）在种子成熟和种子萌发过程中发挥着防御作用。然而，在鹰嘴豆中还没有开展过此类调查。本研究对 LEA 编码基因进行了全基因组鉴定和表征，在 desi 和 kabuli 栽培品种鹰嘴豆中分别鉴定出 65 和 74 个 LEA 编码基因。根据其系统发育分析和保守结构域，所有这些基因被分为八个亚家族。研究发现，LEA 编码基因中最大的成员属于 LEA_2 基因家族。此外，还对 LEA 的理化性质进行了分析。发现 LEA 编码基因位于鹰嘴豆的所有染色体（8 chr）上，并根据基因本体分析确定其参与刺激响应、生物过程、分子功能和细胞成分。随机选取的 8 个 LEA 编码基因在不同的种子发育阶段进行了基因表达分析，结果表明 LEA 编码基因在鹰嘴豆种子发育后期的表达量较高，并证明了它们在种子成熟过程中干燥过程中的潜在作用。在种子萌发过程中，对 LEA 编码基因的表达分析发现其在种子萌发初期的表达量较高。总之，这项工作强调了鹰嘴豆中 LEA 编码基因的全基因组鉴定和特征描述，并提出了它们在种子发育过程中的潜在作用。这些信息也可作为鹰嘴豆分子育种的参考调查，以了解种子的抗逆性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Gene Agricultural and Biological Sciences-Plant Science

CiteScore

4.50

自引率

0.00%

发文量

审稿时长

51 days

期刊介绍： Plant Gene publishes papers that focus on the regulation, expression, function and evolution of genes in plants, algae and other photosynthesizing organisms (e.g., cyanobacteria), and plant-associated microorganisms. Plant Gene strives to be a diverse plant journal and topics in multiple fields will be considered for publication. Although not limited to the following, some general topics include: Gene discovery and characterization, Gene regulation in response to environmental stress (e.g., salinity, drought, etc.), Genetic effects of transposable elements, Genetic control of secondary metabolic pathways and metabolic enzymes. Herbal Medicine - regulation and medicinal properties of plant products, Plant hormonal signaling, Plant evolutionary genetics, molecular evolution, population genetics, and phylogenetics, Profiling of plant gene expression and genetic variation, Plant-microbe interactions (e.g., influence of endophytes on gene expression; horizontal gene transfer studies; etc.), Agricultural genetics - biotechnology and crop improvement.